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Recombinant Human MFAP4 Protein, CF

Catalog # 10230-MF | R&D Systems, Inc. a Bio-Techne Brand
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10230-MF-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human MFAP4 protein
Val22-Ala255, with an N-terminal c-Myc Tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu (c-Myc Tag)

Predicted Molecular Mass

28 kDa

SDS-PAGE

35-45 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human MFAP4 (Catalog # 10230-MF) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Human Fibrillin-1/FBN1 Fc Chimera (Catalog # 10224-FI) nbsp; that produces 50% of the optimal binding response is 0.1-0.6 μg/mL.

Scientific Data Images for Recombinant Human MFAP4 Protein, CF

Recombinant Human MFAP4 Protein Binding Activity

Recombinant Human MFAP4 Protein Binding Activity

When Recombinant Human MFAP4 (Catalog # 10230-MF) is coated at 0.5 µg/mL, 100 µL/well, Recombinant Human Fibrillin-1/FBN1 Fc Chimera (Catalog # 10224-FI) binds with an ED50 of 0.1-0.6 µg/mL.
Recombinant Human MFAP4 Protein SDS-PAGE

Recombinant Human MFAP4 Protein SDS-PAGE

2 μg/lane of Recombinant Human MFAP4 (Catalog # 10230-MF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-45 kDa and 70-90 kDa, respectively.

Formulation, Preparation and Storage

10230-MF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MFAP4

Microfibrillar-associated protein 4 (MFAP4) is an extracellular glycoprotein found in elastic fibers that may support elastic fiber maintenance and assembly (1). It contains fibrinogen-like domains and an RGD sequence in the N-terminus that serves as the ligand motif for the cell receptor integrin (2). MFAP4 exists as a disulfide-linked dimer that is able to form higher oligomeric structures (3). Mature human FAP4 shares 95% amino acid sequence identity with mouse and bovine MFAP4. Binding analysis revealed that MFAP4 specifically binds tropoelastin and firillin-1 and -2, as well as the elastin cross-linking amino acid desmosine, and it co‑localizes with fibrillin-1 positive fibers in vivo (1). MFAP4 contributes to the development of elastic fiber and may play a potential role in extracellular matrix (ECM) turnover during fibrogenesis (2, 4). Immunohistochemical studies showed that MFAP4 is highly abundant in hepatic stellate cells and cirrhotic ECM, but not in hepatic parenchyma or normal liver (4). MFAP4 interacts with different collectins in the lung and might fix them in the ECM during inflammation (5). Serum MFAP4 concentrations increased with progressive stages (fibrosis stage 0-4) of hepatic fibrosis in patients and has been proposed as a biomarker for hepatic fibrosis (6, 7).

References

  1. Pilecki, B. et al. (2016) J. Biol. Chem. 291:1103.
  2. Gressner, O.A. et al. (2007) J. Cell Mol. Med. 11:1031.
  3. Schlosser A. et al. (2006) Scand J Immunol. 64:104.
  4. Hunes RO. (1992) Cell. 69:11.
  5. Kasamatsu, S. et al. (2011) Sci. Rep. 1:164.
  6. Lausen, M. et al. (2017) J. Biol. Chem. 274:32234.
  7. Molleken, C. et al. (2009) Hepatology. 49:1257.

Long Name

Microfibril-associated Glycoprotein 4

Alternate Names

MFAP-4

Entrez Gene IDs

4239 (Human); 76293 (Mouse)

Gene Symbol

MFAP4

UniProt

Product Documents for Recombinant Human MFAP4 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human MFAP4 Protein, CF

For research use only

Reconstitution Calculator

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