Product Specifications for Recombinant Human MAN1A1 His-tag Protein, CF
Chinese Hamster Ovary cell line, CHO-derived human MAN1A1 protein Pro63-Glu653, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
63-65 kDa, under reducing conditions.
Measured by its ability to remove alpha -mannose from the high mannose glycan Man-9. A distinct band is observed in the rhMAN1A1 digested sample on SDS-PAGE gel, as measured under the described conditions.
Scientific Data Examples for Recombinant Human MAN1A1 His-tag Protein, CF
Recombinant Human MAN1A1 His-tag Protein Enzyme Activity Diagram.
MAN1A1 is involved in the removal of 4 distinct alpha -1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2, an essential step of N-glycan maturation.
Recombinant Human MAN1A1 His-tag Protein SDS-PAGE.
1 μg/lane of Recombinant Human MAN1A1 His-tag (Catalog # 10665-GT) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 63-65 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after opening.
N-glycan maturation in Golgi apparatus starts with high-mannose glycan Man-9 that is capped with four 1,2-alpha -linked mannose residues at its non-reducing ends. During the process, these mannose residues are removed to generate Man-5 oligomannose glycan, a precursor for complex and hybrid N-glycans (1). Failure of removing these mannose residues will result in the display of high-mannose glycans on cell surface and extracellular matrix. Increased levels of high-mannose glycans on cell surface are usually associated with disease progress such as tumorigenesis and viral infection (2). The removal of 1,2-alpha -linked mannose residues are catalyzed by 4 alpha -mannosidases, including MAN1A1, MAN1B1, MAN1A2 and MAN1C1, that have overlapping substrate specificity and slight differences in enzyme activity (3). MAN1A1 is also a tumor-suppressor (4) and low levels of expression of MAN1A1 correlate with poor prognosis in breast cancer patients (5, 6).
Oliveira-Ferrer, L. et al. (2014) Br. J. Cancer 110:753.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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