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Recombinant Human MAN1A1 His-tag Protein, CF

Catalog # 10665-GH | R&D Systems, Inc. a Bio-Techne Brand
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10665-GH-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human MAN1A1 protein
Pro63-Glu653, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro63

Predicted Molecular Mass

67.7 kDa

SDS-PAGE

63-65 kDa, under reducing conditions.

Activity

Measured by its ability to remove alpha-mannose from the high mannose glycan Man-9.
A distinct band is observed in the rhMAN1A1 digested sample on SDS-PAGE gel, as measured under the described conditions.

Scientific Data Images for Recombinant Human MAN1A1 His-tag Protein, CF

Recombinant Human MAN1A1 His-tag Protein Enzyme Activity Diagram.

MAN1A1 is involved in the removal of 4 distinct alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2, an essential step of N-glycan maturation.

Recombinant Human MAN1A1 His-tag Protein SDS-PAGE.

1 μg/lane of Recombinant Human MAN1A1 His-tag (Catalog # 10665-GT) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 63-65 kDa.

Formulation, Preparation and Storage

10665-GH
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: MAN1A1

N-glycan maturation in Golgi apparatus starts with high-mannose glycan Man-9 that is capped with four 1,2-alpha -linked mannose residues at its non-reducing ends. During the process, these mannose residues are removed to generate Man-5 oligomannose glycan, a precursor for complex and hybrid N-glycans (1). Failure of removing these mannose residues will result in the display of high-mannose glycans on cell surface and extracellular matrix. Increased levels of high-mannose glycans on cell surface are usually associated with disease progress such as tumorigenesis and viral infection (2). The removal of 1,2-alpha -linked mannose residues are catalyzed by 4 alpha-mannosidases, including MAN1A1, MAN1B1, MAN1A2 and MAN1C1, that have overlapping substrate specificity and slight differences in enzyme activity (3). MAN1A1 is also a tumor-suppressor (4) and low levels of expression of MAN1A1 correlate with poor prognosis in breast cancer patients (5, 6).

References

  1. Oliveira-Ferrer, L. et al. (2014) Br. J. Cancer 110:753.
  2. Moremen K.W. et al. (2012) Nat. Rev. Mol. Cell Biol. 13:448.
  3. Moremen, K.W. and Nairn, A.V. (2014) Handbook of Glycosyltransferases and Related Genes p1297.
  4. Liu, T. et al. (2014) PLoS One 9:e107941.
  5. Milde-Langosch, K. et al. (2014) Breast Cancer Res. Treat. 145:295.
  6. Karen Legler, et al. (2018) Br. J. Cancer 118:847.

Long Name

Mannosidase alpha, Class 1A, Member 1

Alternate Names

HUMM3, HUMM9, MAN9

Entrez Gene IDs

4121 (Human); 17155 (Mouse)

Gene Symbol

MAN1A1

UniProt

Product Documents for Recombinant Human MAN1A1 His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human MAN1A1 His-tag Protein, CF

For research use only

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