Source	Chinese Hamster Ovary cell line, CHO-derived human MAN1A1 protein Pro63-Glu653, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Pro63
Predicted Molecular Mass	67.7 kDa
SDS-PAGE	63-65 kDa, under reducing conditions
Activity	Measured by its ability to remove alpha -mannose from the high mannose glycan Man-9. A distinct band is observed in the rhMAN1A1 digested sample on SDS-PAGE gel, as measured under the described conditions.

Recombinant Human MAN1A1 His-tag Protein, CF Scientific Data Examples

	Recombinant Human MAN1A1 His-tag Protein Enzyme Activity Diagram. MAN1A1 is involved in the removal of 4 distinct alpha -1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2, an essential step of N-glycan maturation.
	Recombinant Human MAN1A1 His-tag Protein SDS-PAGE. 1 μg/lane of Recombinant Human MAN1A1 His-tag (Catalog # 10665-GT) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 63-65 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10665-GH

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl₂.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: MAN1A1

N-glycan maturation in Golgi apparatus starts with high-mannose glycan Man-9 that is capped with four 1,2-alpha -linked mannose residues at its non-reducing ends. During the process, these mannose residues are removed to generate Man-5 oligomannose glycan, a precursor for complex and hybrid N-glycans (1). Failure of removing these mannose residues will result in the display of high-mannose glycans on cell surface and extracellular matrix. Increased levels of high-mannose glycans on cell surface are usually associated with disease progress such as tumorigenesis and viral infection (2). The removal of 1,2-alpha -linked mannose residues are catalyzed by 4 alpha -mannosidases, including MAN1A1, MAN1B1, MAN1A2 and MAN1C1, that have overlapping substrate specificity and slight differences in enzyme activity (3). MAN1A1 is also a tumor-suppressor (4) and low levels of expression of MAN1A1 correlate with poor prognosis in breast cancer patients (5, 6).

Item	Value
References	Oliveira-Ferrer, L. et al. (2014) Br. J. Cancer 110:753. Moremen K.W. et al. (2012) Nat. Rev. Mol. Cell Biol. 13:448. Moremen, K.W. and Nairn, A.V. (2014) Handbook of Glycosyltransferases and Related Genes p1297. Liu, T. et al. (2014) PLoS One 9:e107941. Milde-Langosch, K. et al. (2014) Breast Cancer Res. Treat. 145:295. Karen Legler, et al. (2018) Br. J. Cancer 118:847.
Long Name	Mannosidase alpha, Class 1A, Member 1
Entrez Gene IDs	4121 (Human); 17155 (Mouse)
Alternate Names	HUMM3, HUMM9, MAN1A1, MAN9

Citations for Recombinant Human MAN1A1 His-tag Protein, CF

View all citations for the Proteins and Enzymes platform.
Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Recombinant Human MAN1A1 His-tag Protein, CF

Order Information

Shipping Information

Key Product Details

Complete Your Research

Product Specifications