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Recombinant Human LTK Protein, CF

Bio-Techne includes R&D Systems | Catalog # 9376-LK

Catalog #
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Key Product Details



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Product Specifications


Mouse myeloma cell line, NS0-derived human LTK protein
Ser20-Pro424, with a C-terminal His-tag


>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis


Predicted Molecular Mass

42 kDa


56-84 kDa, reducing conditions


Measured by its binding ability in a functional ELISA.
When Recombinant Human LTK is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human FAM-150A that produces 50% of the optimal binding response is 40-240 ng/mL.

Scientific Data Images for Recombinant Human LTK Protein, CF

Recombinant Human LTK Protein Bioactivity

Recombinant Human LTK Protein Bioactivity

When Recombinant Human LTK (Catalog # 9376-LK) is immobilized at 2 μg/mL, 100 μL/well, Recombinant Human FAM-150A binds with an ED50 of 40-240 ng/mL.

Formulation, Preparation and Storage

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LTK

LTK, also known as Leukocyte tyrosine kinase receptor and protein tyrosine kinase 1, is a type I transmembrane protein and shares a high degree of sequence similarity with kinases in the insulin receptor family (1). Alternative splicing generates several isoforms of 50-100 kDa (2). LTK contains a 408 aa extracellular domain, 25 aa transmembrane domain, and 415 aa cytoplasmic domain. The cytoplasmic region has several phosphotyrosines that interact with downstream signaling molecules (3). LTK is reported to undergo dimerand trimerization when under certain conditions. The extracellular domain of human LTK shares 75% homology with mouse LTK. It is expressed in preB lymphocytes, B lymphocytes, and other hematopoietic cells, as well as brain and placenta (48). Human LTK can bind to PLCgamma 1, PI3K, GAP and Raf1 in vivo (6). LTK promotes neurite outgrowth via PI3K/AKT and Ras/MAPK pathways (7). FAM150A and FAM150B can both bind to the extracellular domain of LTK, but only FAM150A is the specific ligand for LTK (9, 10).


  1. Honda, H. et al. (1999) Oncogene 18:3821.
  2. Yazaki, Y. et al. (1993) Proc Natl. Acad. Sci. U.S.A. 90:5404.
  3. Maru, Y., et al. (1990) Oncogene Res. 5:19.
  4. Ben-Neriah, Y. et al. (1988) Nature 333:672.
  5. Bernards, A. et al. (1990) EMBO J. 9:2279.
  6. Kozutsumi, H et al. (1994) Oncogene 9:2991.
  7. Yamada, S. et al. (2008) Neuroreport 19:1733.
  8. Roll, JD. et al. (2012) PLoS One 7:e31733.
  9. Reshetnyak, AV. et al. (2015) Proc Natl Acad Sci U S A. 112:15862.
  10. Guan, J, AV. et al. (2015) Elife. 4:e09811.

Long Name

Leukocyte Tyrosine Kinase Receptor

Alternate Names


Entrez Gene IDs

4058 (Human); 17005 (Mouse); 311337 (Rat)

Gene Symbol



Additional LTK Products

Product Documents for Recombinant Human LTK Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human LTK Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.