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Recombinant Human LRRTM1 Fc Chimera Protein, CF

Catalog # 10372-LR | R&D Systems, Inc. a Bio-Techne Brand
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10372-LR-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human LRRTM1 protein
Human LRRTM1
(Ala35-Ile425)
Accession # Q86UE6.2
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala35

Predicted Molecular Mass

70 kDa

SDS-PAGE

99-109 kDa, under reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human LRRTM1 Fc Chimera (Catalog # 10372-LR), immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth

Scientific Data Images for Recombinant Human LRRTM1 Fc Chimera Protein, CF

Recombinant Human LRRTM1 Fc Chimera Protein SDS-PAGE

Recombinant Human LRRTM1 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human LRRTM1 Fc Chimera (Catalog # 10372-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 99-109 and 190-210 kDa, respectively.

Formulation, Preparation and Storage

10372-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRTM1

Human LRRTM1 (leucine-rich repeat transmembrane neuronal 1) is a 58-59 kDa (predicted) type I transmembrane protein, that belongs to the LRRTM family of proteins within the leucine-rich repeat (LRR) superfamily (1). It is synthesized as a precursor with a 34 amino acid (aa) signal sequence, a 393 aa luminal region, a 21 aa transmembrane region, and a 74 aa cytoplasmic region. The luminal portion of LRRTM1 contains three N-linked glycosylation sites and 10 LRRs flanked by cysteine-rich domains (1). The cytoplasmic region contains several tyrosine, serine, and threonine residues that have potential to be phosphorylated and thus to be involved in signal transduction (1). The C-terminal also contains a conserved glutamic acid-cysteine-glutamic acid-valine sequence for potential interaction with PDZ proteins (1-2). Mature human LRRTM1 is 97% aa identical to mouse LRRTM1. LRRTM1 is expressed in both developing and adult brains. It can be detected in many brain areas such the forebrain, mid brain, olfactory bulb, striatum, thalamus and cerebral cortex (3). LRRTM1 proteins are mainly localized to postsynaptic sites and bind across the synaptic cleft to its presynaptic partner neurexins lacking the splice site 4 insert (3, 4). LRRTM1 functions as a synapse organizer and plays an essential role in synapse formation and development (3, 4). Functionally, LRRTM1 may be involved in the formation of the CNS and maintenance of CNS structure and function in the adult brain (1). In addition, LRRTM1 has been shown to be a maternally suppressed gene that is associated paternally with handedness and schizophrenia (3). LRRTM1 was also reported to be associated with schizophrenia and autism spectrum disorder (5, 6).

References

  1. Lauren, J. et al. (2003) Genomics 81:411.
  2. Haines, B.P. and P.W.J. Rigby (2007) Gene Expr. Patterns 7:23.
  3. Roppongi, R.T. et al. (2017) Neurosci. Res. 116:18.
  4. Ko, J. (2012) Mol. Cells. 34:335.
  5. Francks, C. et al. (2007) Mol. Psychiatry 12:1129.
  6. Sousa, I. et al. (2010) Mol. Autism 1:17.

Long Name

Leucine Rich Repeat Transmembrane Neuronal 1

Alternate Names

FLJ32082, leucine rich repeat transmembrane neuronal 1, leucine-rich repeat transmembrane neuronal 1 protein, leucine-rich repeat transmembrane neuronal protein 1

Entrez Gene IDs

347730 (Human); 74342 (Mouse); 679668 (Rat)

Gene Symbol

LRRTM1

UniProt

Additional LRRTM1 Products

Product Documents for Recombinant Human LRRTM1 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human LRRTM1 Fc Chimera Protein, CF

For research use only

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