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Recombinant Human LR3 IGF-I/IGF-1 GMP Protein, CFGMP


Order Information
Key Product Details
Source: | E. coli |
Accession #: | P05019 |
Applications: | Bioactivity |
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Product Specifications
Source |
E. coli-derived human IGF-I/IGF-1 protein
Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidelines. |
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Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. The result of the major peak from mass spectrometry analysis is 9111 Da, which corresponds to the calculated molecular mass of 9118 Da. | ||||
Endotoxin level | <0.01 EU per 1 μg of the protein by the LAL method. | ||||
N-terminal sequence Analysis | Met-Phe-Pro-Ala-Met-Pro-Leu-Ser-Ser-Leu | ||||
Predicted Molecular Mass | 9 kDa | ||||
SDS-PAGE | 8 kDa, reducing conditions | ||||
Activity | Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.3-1.5 ng/mL. IGFBP-3 does not inhibit its activity. |
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GMP-grade Recombinant Human LR3 IGF-I/IGF-1 (Catalog # 8335D‑GMP) stimulates cell proliferation in a serum-free assay using the MCF‑7 human breast cancer cell line. The ED50for this effect is 0.3‑1.5 ng/mL. |
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2 μg/lane of GMP-grade Recombinant Human LR3 IGF-I/IGF-1 (Catalog # 8335D-GMP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing R and NR bands at 8 kDa. |
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier Free
Formulation | Lyophilized from a 0.2 μm filtered solution in Acetic Acid. |
Reconstitution | Reconstitute at 100-200 μg/mL in sterile 100 mM Acetic Acid |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: IGF-I/IGF-1
Long R3 IGF-1 (LR3 IGF-1) is a 9.2 kDa synthetic analog of IGF-1 that is generated by modifying the aa sequence for mature human IGF-1. These modifications include the substitution of an Arg for Glu at position 3 of the mature IGF-1 sequence and the addition of a thirteen aa N-terminal extension, which is derived from methionyl porcine Growth Hormone (17). These aa changes generate a protein that is still capable of binding to IGF-1 and Insulin receptors, but shows considerably lower affinity binding to IGFBPs compared to wild-type IGF-1 (17, 18). As a result, LR3 IGF-1 has an increased half-life and displays increased biological potency compared to IGF-1 (17-22).
Item | Value |
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References |
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Long Name | Insulin-like Growth Factor I/Insulin-like Growth Factor 1 |
Entrez Gene IDs | 3479 (Human); 16000 (Mouse); 24482 (Rat) |
Alternate Names | IBP1, IGF-1, IGF-I, IGF-IA, IGF-IB, IGF1, IGF1A, IGFI, MGF, Mechano growth factor, Somatomedin A, Somatomedin C, insulin-like growth factor 1, insulin-like growth factor 1 (somatomedin C), insulin-like growth factor I, insulin-like growth factor IA, insulin-like growth factor IB, somatomedin-C |
Citations
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