Recombinant Human Integrin alpha V beta 6 Protein, CF Best Seller

R&D Systems, part of Bio-Techne | Catalog # 3817-AV

R&D Systems, part of Bio-Techne
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3817-AV-050
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Key Product Details

Source

CHO

Accession #

NP_002201.1 (Integrin alphaV) & P18564 (Integrin beta6)

Structure / Form

Noncovalently-linked heterodimer

Conjugate

Unconjugated

Applications

Binding Activity

View all Integrin alpha V beta 6 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha V beta 6 protein
Human Integrin alphaV
(Phe31-Val992)
Accession # NP_002201.1		 His-Pro GGGSGGGS Acidic Tail
Human Integrin beta6
(Gly22-Asn707)
Accession # P18564		 His-His-Pro GGGSGGGS Basic Tail
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe31 ( alphaV subunit) & Gly22 ( beta6 subunit)

Predicted Molecular Mass

110.5 kDa ( alphaV subunit), 78.6 kDa ( beta6 subunit)

SDS-PAGE

118-144 kDa and 93-113 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Human Integrin  alphaV beta6 at 2.0 µg/mL can bind Recombinant Human LAP TGF‑ beta1 (Catalog # 246-LP) with an apparent Kd <0.1 nM.

Reviewed Applications

Read 2 reviews rated 5 using 3817-AV in the following applications:

Formulation, Preparation and Storage

3817-AV
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha V beta 6

Integrin alphaV beta6 is one of five alphaV integrins and the sole beta6 integrin (1, 2). The non-covalent heterodimer of 170 kDa alphaV/CD51 and 95 kDa beta6 integrin subunits is expressed exclusively on subsets of epithelial cells, especially during development, after injury or inflammation, or on many carcinomas (2-5). The ligand interaction site of alphaV beta6 is in the N-terminal head region formed by an interaction of the beta6 vWFA domain with the alphaV beta-propeller structure (2). The alphaV subunit contains domains termed thigh, calf, and calf-2 with a divalent cation-binding site found at a position equivalent to the “knee”. The 962 aa human alphaV ECD (4), which is cleaved at aa 890 but remains associated, shares 92-95% aa sequence identity with mouse and bovine alphaV, while the 685 aa human beta6 ECD (5) shares 90-93% aa sequence identity with mouse, rat, bovine, ovine, and porcine beta6. Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. The beta6 C-terminal 11 amino acid (aa) cytoplasmic sequence transduces a signal, enhancing proliferation and inducing MMP-9 expression (6). Either “inside-out” signaling or Mg2+ or Mn2+ binding unfolds and activates the integrin (1). Active alphaV beta6 binds matrix proteins fibronectin and tenascin C (2). It also binds the TGF-beta latency‑associated peptide (LAP) and activates TGF-beta 1 or TGF-beta 3 from large latent complexes (7). This activation requires interaction with LTBP-1 and fibronectin, and is enhanced by PAR-1 (8, 9). Deletion of beta6 ablates tonic inhibition of alveolar macrophages by TGF-beta, inhibits intestinal regulatory T cell production, and predisposes mice to inflammatory reactions in the skin, lungs, and intestines where irritations and microbial challenges are frequent (10-12). High alphaV beta6 expression in carcinomas may contribute to progression through its effects on TGF-beta and MMP activity (3). The foot-and-mouth disease virus and several other viruses can use alphaV beta6 as a receptor, and soluble alphaV beta6 may block virus infectivity in vitro (13, 14).

References

  1. Hynes, R.O. (2002) Cell 110:673.
  2. Sheppard, D. (2004) Curr. Opin. Cell Biol. 16:552.
  3. Bandyopadhyay, A. and S. Raghavan (2009) Curr. Drug Targets 10:645.
  4. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14080.
  5. Sheppard, D. et al. (1990) J. Biol. Chem. 265:11502.
  6. Dixit, R.B. et al. (1996) J. Biol. Chem. 271:25976.
  7. Munger, J.S. et al. (1999) Cell 96:319.
  8. Fontana, L. et al. (2005) FASEB J. 19:1798.
  9. Jenkins, R.G. et al. (2006) J. Clin. Invest. 116:1606.
  10. Huang, X.Z. et al. (1996) J. Cell Biol. 133:921.
  11. Morris, D.G. et al. (2003) Nature 422:169.
  12. Chen, X. et al. (2011) J. Leukoc. Biol. 90:751.
  13. Berryman, S. et al. (2005) J. Virol. 79:8519.
  14. Heikkila, O. et al. (2009) J. Gen. Virol. 90:197.

Alternate Names

CD51, integrin subunit alpha V, ITGAV, MSK8, VNRA, VTNR

Entrez Gene IDs

3685 (Human)

Gene Symbol

ITGAV

UniProt

NP_002201.1 (Integrin alphaV) & P18564 (Integrin beta6)

Additional Integrin alpha V beta 6 Products

Product Documents for Recombinant Human Integrin alpha V beta 6 Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Human Integrin alpha V beta 6 Protein, CF

For research use only

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