Recombinant Human Integrin alpha V beta 6 Protein, CF Best Seller

Catalog # 3817-AV | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #

NP_002201.1 (Integrin alphaV) & P18564 (Integrin beta6)

Source

CHO

Structure / Form

Noncovalently-linked heterodimer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha V beta 6 protein
Human Integrin alphaV
(Phe31-Val992)
Accession # NP_002201.1		 His-Pro GGGSGGGS Acidic Tail
Human Integrin beta6
(Gly22-Asn707)
Accession # P18564		 His-His-Pro GGGSGGGS Basic Tail
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe31 ( alphaV subunit) & Gly22 ( beta6 subunit)

Predicted Molecular Mass

110.5 kDa ( alphaV subunit), 78.6 kDa ( beta6 subunit)

SDS-PAGE

145 kDa and 115 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Human Integrin  alphaV beta6 at 2.0 µg/mL can bind Recombinant Human LAP TGF‑ beta1 (Catalog # 246-LP) with an apparent Kd

Formulation, Preparation and Storage

3817-AV
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha V beta 6

Integrin alphaV beta6 is one of five alphaV integrins and the sole beta6 integrin (1, 2). The non-covalent heterodimer of 170 kDa alphaV/CD51 and 95 kDa beta6 integrin subunits is expressed exclusively on subsets of epithelial cells, especially during development, after injury or inflammation, or on many carcinomas (2-5). The ligand interaction site of alphaV beta6 is in the N-terminal head region formed by an interaction of the beta6 vWFA domain with the alphaV beta-propeller structure (2). The alphaV subunit contains domains termed thigh, calf, and calf-2 with a divalent cation-binding site found at a position equivalent to the “knee”. The 962 aa human alphaV ECD (4), which is cleaved at aa 890 but remains associated, shares 92-95% aa sequence identity with mouse and bovine alphaV, while the 685 aa human beta6 ECD (5) shares 90-93% aa sequence identity with mouse, rat, bovine, ovine, and porcine beta6. Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. The beta6 C-terminal 11 amino acid (aa) cytoplasmic sequence transduces a signal, enhancing proliferation and inducing MMP-9 expression (6). Either “inside-out” signaling or Mg2+ or Mn2+ binding unfolds and activates the integrin (1). Active alphaV beta6 binds matrix proteins fibronectin and tenascin C (2). It also binds the TGF-beta latency‑associated peptide (LAP) and activates TGF-beta 1 or TGF-beta 3 from large latent complexes (7). This activation requires interaction with LTBP-1 and fibronectin, and is enhanced by PAR-1 (8, 9). Deletion of beta6 ablates tonic inhibition of alveolar macrophages by TGF-beta, inhibits intestinal regulatory T cell production, and predisposes mice to inflammatory reactions in the skin, lungs, and intestines where irritations and microbial challenges are frequent (10-12). High alphaV beta6 expression in carcinomas may contribute to progression through its effects on TGF-beta and MMP activity (3). The foot-and-mouth disease virus and several other viruses can use alphaV beta6 as a receptor, and soluble alphaV beta6 may block virus infectivity in vitro (13, 14).

References

  1. Hynes, R.O. (2002) Cell 110:673.
  2. Sheppard, D. (2004) Curr. Opin. Cell Biol. 16:552.
  3. Bandyopadhyay, A. and S. Raghavan (2009) Curr. Drug Targets 10:645.
  4. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14080.
  5. Sheppard, D. et al. (1990) J. Biol. Chem. 265:11502.
  6. Dixit, R.B. et al. (1996) J. Biol. Chem. 271:25976.
  7. Munger, J.S. et al. (1999) Cell 96:319.
  8. Fontana, L. et al. (2005) FASEB J. 19:1798.
  9. Jenkins, R.G. et al. (2006) J. Clin. Invest. 116:1606.
  10. Huang, X.Z. et al. (1996) J. Cell Biol. 133:921.
  11. Morris, D.G. et al. (2003) Nature 422:169.
  12. Chen, X. et al. (2011) J. Leukoc. Biol. 90:751.
  13. Berryman, S. et al. (2005) J. Virol. 79:8519.
  14. Heikkila, O. et al. (2009) J. Gen. Virol. 90:197.

Alternate Names

Integrin alpha V beta 6, ITGAV, CD51, integrin subunit alpha V, MSK8, VNRA, VTNR

Entrez Gene IDs

3685 (Human)

Gene Symbol

ITGAV

UniProt

NP_002201.1 (Integrin alphaV) & P18564 (Integrin beta6)

Product Documents for Recombinant Human Integrin alpha V beta 6 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Integrin alpha V beta 6 Protein, CF

For research use only

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FAQs for Recombinant Human Integrin alpha V beta 6 Protein, CF

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  • Q: What is the amino acid sequence of the acidic and basic tails?

    A: Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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