Recombinant Human Integrin alpha 3 beta 1/VLA-3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2840-A3

R&D Systems, part of Bio-Techne
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2840-A3-050
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Key Product Details

Source

CHO

Accession #

P26006 (Integrin alpha3) & P05556 (Integrin beta1)

Conjugate

Unconjugated

Applications

Binding Activity

View all Integrin alpha 3 beta 1/VLA-3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 3 beta 1/VLA-3 protein
Human Integrin alpha3
(Phe33-Glu991)
Accession # P26006		 acidic tail
Human Integrin beta1
(Glu21-Asp728)
Accession # P05556		 basic tail
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe33 (Integrin alpha3) & No results obtained: Gln21 predicted (Integrin beta1)

Predicted Molecular Mass

110.5 kDa (Integrin alpha3), 82.3 kDa (Integrin beta1)

SDS-PAGE

150 kDa and 125 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized Collagen IV at 10 µg/mL can bind rhIntegrin a3/ beta1 with an apparent Kd <2 nM.

Formulation, Preparation and Storage

2840-A3
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 3 beta 1/VLA-3

VLA-3 (Very Late Antigen 3) is a member of the integrin family, beta1 subfamily, of cell membrane adhesion molecules (1-3). Integrins are nondisulfide-linked transmembrane (TM) heterodimers that contain an alpha- and beta-subunit (1). VLA-3 is composed of an alpha3 and beta1 subunit. The alpha3/CD49c subunit is a 130-150 kDa type I TM glycoprotein. It only associates with the beta1 integrin subunit. It is synthesized as a 1051 amino acid (aa) precursor that undergoes proteolytic cleavage to generate a disulfide-linked 110 kDa, 843 aa extracellular heavy chain and a 30 kDa, 176 aa TM/cytoplasmic light chain (1, 4, 5, 6). The heavy chain contains seven 60 aa repeats that fold into a propeller-like structure (7). Sequences involving the first three repeats are associated with ligand binding (1). The light chain has two cytoplasmic alternate splice forms. The A form cytoplasmic domain is 52 aa, while the B form cytoplasmic domain is 37 aa (5). Human alpha3 heavy chain is 88% aa identical to mouse heavy chain. The human beta1/CD29 subunit is a 130-140 kDa type I transmembrane glycoprotein (8). Unlike the alpha-subunit, it does not undergo proteolytic cleavage. The molecule contains a 708 aa extracellular region, a 23 aa TM segment, and a 47 aa cytoplasmic domain. The extracellular region contains one von Willebrand factor-like A domain and four cysteine-rich repeats. The cytoplasimc domain shows five alternate splice forms (A, B, C1, C2 and D), all which vary by 12 to 48 amino acids (9, 10). Although the functions of all are unclear, the B isoform appears to be dominant negative to the A isoform (11). Human beta1 extracellular domain is 92%, 94%, 93% and 96% aa identical to the extracellular regions of beta1 in rat, bovine, mouse and feline, respectively. VLA-3 is known to bind fibronectin, collagen, and laminin-1, 5, 8, 10 and 11 (1). It also binds tetraspanins such as CD9, CD63 and CD151. CD151 binding may actually stabilize VLA-3, enabling it to bind to additional factors (12).

References

  1. Tsuji, T. et al. (2004) J. Membr. Biol. 200:115.
  2. Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
  3. Kreidberg, J.A. (2000) Curr. Opin. Cell Biol. 12:548.
  4. Takada, Y. et al. (1991) J. Cell. Biol. 115:257.
  5. de Melker, A.A. et al. (1997) Lab. Invest. 76:547.
  6. Krokhin, O.V. et al. (2003) Biochemistry 42:12950.
  7. Springer, T.A. (2002) Curr. Opin. Struct. Biol. 12:802.
  8. Argraves, W.S. et al. (1987) J. Cell Biol. 105:1183.
  9. Svineng, G. et al. (1998) Biochem. J. 330:1255.
  10. van der Flier, A. et al. (1995) FEBS Lett. 369:340.
  11. Balzac, F. et al. (1994) J. Cell Biol. 127:557.
  12. Nishiuchi, R. et al. (2005) Proc. Natl. Acad. Sci. USA 102:1939.

Alternate Names

VLA-3, VLA3

Entrez Gene IDs

3675 (Human)

Gene Symbol

ITGA3

UniProt

P26006 (Integrin alpha3) & P05556 (Integrin beta1)

Additional Integrin alpha 3 beta 1/VLA-3 Products

Product Documents for Recombinant Human Integrin alpha 3 beta 1/VLA-3 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Integrin alpha 3 beta 1/VLA-3 Protein, CF

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