Product Specifications for Recombinant Human IL-9 Protein, CF
Human embryonic kidney cell, HEK293-derived human IL-9 protein Gln19-Ile144
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Gln19 inferred from enzymatic pyroglutamate treatment revealing Gly20
Predicted Molecular Mass
26-38 kDa, under reducing conditions.
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 0.200-1.00 ng/mL
Scientific Data Examples for Recombinant Human IL-9 Protein, CF
Recombinant Human IL‑9 Protein Bioactivity.
Recombinant Human IL-9 (Catalog # 11081-IL) induces MO7e human megakaryocytic leukemic cell proliferation. The ED50 for this effect is 0.200‑1.00 ng/mL
Recombinant Human IL‑9 Protein SDS-PAGE.
2 μg/lane of Recombinant Human IL‑9 Protein (Catalog # 11081-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 26-38 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 200 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin-9 (IL-9), also known as P40 and MEA (mast cell growth-enhancing activity), is a 30-40 kDa glycosylated member of a cytokine family that includes Interleukins-2, -4, -7, -15, and -21. These proteins utilize heteromeric receptors containing the Common gamma chain ( gamma c) in addition to ligand-specific subunits. IL-9 interacts selectively with IL-9 R which then associates with gamma c to form the functional receptor complex. IL-9 contributes to allergic inflammation, autoimmunity-induced inflammation, parasite clearance from the GI tract, and Treg-mediated immune suppression (1, 2). It enhances the expansion and recruitment of mast cells and eosinophils as well as the production of IgE and Th2 cytokines (3‑6). It is required for anaphylactic responses to ingested allergens but not to systemic allergens (7). IL-9 plays multiple roles in the development and function of subsets within the CD4+ T cell lineage (8). It is expressed by activated Th9, Th17, Treg, and Th2 cells (3, 9‑12). IL-9 acts as an autocrine growth and activation factor for Th17, Treg, and mast cells (3, 11, 13). It also can inhibit immune responses by enhancing the suppressive properties of Treg and by recruiting immune-suppressive mast cells to sites of inflammation (11, 12). Mature human IL-9 shares 57% amino acid sequence identity with mouse and rat IL-9 (14, 15).
Noelle, R.J. and E.C. Nowak (2010) Nat. Rev. Immunol. 10:683.
Goswami, R. and M.H. Kaplan (2011) J. Immunol. 186:3283.
Nowak, E.C. et al. (2009) J. Exp. Med. 206:1653.
Townsend, M.J. et al. (2000) Immunity 13:573.
Leech, M.D. and R.K. Grencis (2006) J. Immunol. 176:2505.
Fawaz, L.M. et al. (2007) J. Allergy Clin. Immunol. 120:1208.
Osterfeld, H. et al. (2010) J. Allergy Clin. Immunol. 125:469.
Jabeen, R. and M.H. Kaplan (2012) Curr. Opin. Immunol. 24:303.
Tan, C. et al. (2010) J. Immunol. 185:6795.
Jager, A. et al. (2009) J. Immunol. 183:7169.
Elyaman, W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:12885.
Lu, L.-F. et al. (2006) Nature 442:997.
Stassen, M. et al. (2000) J. Immunol. 164:5549.
Yang, Y.-C. et al. (1989) Blood 74:1880.
Renauld, J.-C. et al. (1990) Cytokine 2:9.
Entrez Gene IDs
3578 (Human); 16198 (Mouse)
Cytokine P40, HP40, IL-9, IL-9homolog of mouse T cell and mast cell growth factor 40, IL9, P40, T-cell growth factor P40, interleukin 9, interleukin-9, p40 T-cell and mast cell growth factor, p40 cytokine
Citations for Recombinant Human IL-9 Protein, CF
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Product Documents for Recombinant Human IL-9 Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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