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Recombinant Human IL-4 GMP Protein, CFGMP



Order Information
Key Product Details
Source: | E. coli |
Accession #: | P05112 |
Applications: | Bioactivity |
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Product Specifications
Source |
E. coli-derived human IL-4 protein His25-Ser153, with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidelines. |
Purity | >97%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Endotoxin level | <0.10 EU per 1 μg of the protein by the LAL method. |
N-terminal sequence Analysis | Met-His25-Lys-(Cys)-Asp-Ile-Thr-Leu-Gln-Glu |
Predicted Molecular Mass | 15.1 kDa |
SDS-PAGE | 14 kDa, reducing conditions |
Activity |
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.05-0.2 ng/mL. The specific activity of recombinant human IL-4 is >1.0 x 107 IU/mg, which is calibrated against the human IL-4 WHO International Standard (NIBSC code: 88/656). |
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GMP-grade Recombinant Human IL‑4 (Catalog # 204‑GMP) stimulates proliferation of TF‑1 human erythroleukemic cells. The ED50 is 0.05‑0.2 ng/mL. |
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1 μg/lane of GMP-grade Recombinant Human IL-4 (Catalog # 204-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa. |
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MALDI-TOF analysis of GMP-grade Recombinant Human IL-4 (Catalog # 204-GMP). The major peak at 15083 Da corresponds to the calculated molecular mass, 15094 Da. The minor peak at 15297 Da is a matrix-associated artifact of the MALDI-TOF. |
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier Free
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100-200 μg/mL in PBS. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: IL-4
Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5‑7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL‑13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10‑13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).
Item | Value |
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References |
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Long Name | Interleukin 4 |
Entrez Gene IDs | 3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit) |
Alternate Names | B cell growth factor 1, B-cell stimulatory factor 1, BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL-4, IL-4B_cell stimulatory factor 1, IL4, IL4E12, Lymphocyte stimulatory factor 1, MGC79402, binetrakin, interleukin 4, interleukin-4, pitrakinra |
Citations
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