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Recombinant Human IL-3 GMP Protein, CFGMP



Order Information
Key Product Details
Source: | E. coli |
Accession #: | AAC08706 |
Applications: | Bioactivity |
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Product Specifications
Source |
E. coli-derived human IL-3 protein Ala20-Phe152, with and without an N-terminal Met Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidelines. |
Purity | >97%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Endotoxin level | <0.10 EU per 1 μg of the protein by the LAL method. |
N-terminal sequence Analysis |
Met-Ala-Pro-Met-Thr-Gln-Thr-Thr-Ser-Leu Ala-Pro-Met-Thr-Gln-Thr-Thr-Ser-Leu-Lys |
Predicted Molecular Mass | 15 kDa |
Activity |
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.02-0.1 ng/mL. The specific activity of recombinant human IL-3 is >1.7 x 106 IU/mg, which is calibrated against the human IL-3 WHO International Standard (NIBSC code: 91/510). |
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GMP-grade Recombinant Human IL-3 (Catalog # 203-GMP) stimulates proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 0.02-0.1 ng/mL. |
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1 μg/lane of GMP-grade Recombinant Human IL-3 (Catalog # 203-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver stain, showing a single band at 15 kDa. |
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ESI analysis of GMP-grade Recombinant Human IL-3 (Catalog # 203-GMP). The labeled peaks at 15214 Da and 15083 Da correspond to the calculated molecular mass with the N-terminal Met, 15213 Da, and without the N-terminal Met, 15081 Da, respectively. |
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier Free
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100 μg/mL in PBS. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: IL-3
Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and murine IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on murine cells.
IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of at least two subunits, an IL-3 specific alpha chain which binds IL-3 with low affinity and a common beta chain that is shared by the IL-5 and GM-CSF high-affinity receptors. Although the beta chain itself does not bind IL-3, it confers high-affinity IL-3 binding in the presence of the alpha chain. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.
Item | Value |
---|---|
Long Name | Interleukin 3 |
Entrez Gene IDs | 3562 (Human); 16187 (Mouse); 24495 (Rat) |
Alternate Names | Hematopoietic growth factor, IL-3, IL-3MGC79398, IL3, MCGF, MCGFMGC79399, MULTI-CSF, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell stimulating factor, P-cell-stimulating factor, interleukin 3 (colony-stimulating factor, multiple), interleukin-3, mast-cell growth factor, multilineage-colony-stimulating factor |
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