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Recombinant Human IL-29/IFN-lambda 1 Protein, CF

Catalog # 11240-IL | R&D Systems, Inc. a Bio-Techne Brand
E. coli-expressed
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11240-IL-050

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human IL-29/IFN-lambda 1 protein
Pro23-Thr200

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro23

Predicted Molecular Mass

20 kDa

SDS-PAGE

18-22 kDa, under reducing conditions.

Activity

Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
The ED50 for this effect is 0.500-6.00 ng/mL.

Scientific Data Images for Recombinant Human IL-29/IFN-lambda 1 Protein, CF

Recombinant Human IL-29/IFN-lambda 1 Protein Bioactivity.

Recombinant Human IL‑29/IFN‑lambda 1 Protein (Catalog # 11240-IL) demonstrates anti-viral activity in HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.500-6.00 ng/mL.

Formulation, Preparation and Storage

11240-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-29/IFN-lambda 1

IL-29, also known interferon-lambda 1 (IFN lambda1), along with IL-28A (IFN lambda2), IL-28B (IFN lambda3) and IFN lambda4, collectively comprise the type III subset of IFNs. IFN lambdas are class II cytokine receptor ligands distantly related to both members of the IL-10 family and to the type I IFN family (11-19% amino acid (aa) sequence identity). IL-29 is expressed only in humans and shares 67% and 69% aa sequence identity with human IL-28A and IL-28B, respectively. IFN lambda signaling was initially thought to be restricted to epithelial cells, but, recently, additional cell types have been shown to also respond. IFN lambdas have been shown to be induced by a variety of stimuli from influenza A and herpes viruses to lipopolysaccharides and double-stranded RNA. IL-29 signals through a unique receptor complex composed of IFN lambdaR1 (IL-28RA) and the shared IL-10R2 chain, which is also a part of the receptor complexes for IL-10, IL-22, and IL-26. In humans, IL-29 is the most potent IFN lambda molecule, the most abundant IFN lambda molecule in serum, and the only IFN lambda molecule to display N-linked glycosylation. IL-29 is being investigated for its role in immunity, both innate and adaptive, as well as in autoimmune disorders. Additionally, the therapeutic potential of IL-29 in inflammatory diseases and its cancer fighting properties are being actively studied.

References

  1. Vilcek, J. (2003) Nature Immunol. 4:8.
  2. Sheppard, P. et al. (2003) Nature Immunol. 4:63.
  3. Kotenko, S.V. et al. (2003) Nature Immunol. 4:69.
  4. Gad, H.H. et al. (2009) J. Biol. Chem. (2009) 284:20869.
  5. Miknis, Z.J. et al. (2010) J. Mol. Biol. 404:650.
  6. Donnelly, R.P. et al. (2010) J. Interferon. Cytokine Res. 30:555.
  7. Lazear, H.M. et al. (2015) Immunity. 43:15.
  8. Ye, L. et al. (2019) Nat. Rev. Immunol. 19:614.
  9. Goel, R.R. et al. (2021) Nat. Rev. Rheumatol. 17:349.
  10. Kelm, N.E. et al. (2016) Crit. Rev. Oncol. Hematol. 106:91.
  11. Wang, J.M. et al. (2019) J. Cell Mol. Med. 23:7926.

Long Name

Interleukin 29

Alternate Names

IFN-lambda 1, IL29

Entrez Gene IDs

282618 (Human)

Gene Symbol

IFNL1

UniProt

Product Documents for Recombinant Human IL-29/IFN-lambda 1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IL-29/IFN-lambda 1 Protein, CF

For research use only

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