Recombinant Human IL-21 GMP Protein, CFGMP

Name: Recombinant Human IL-21 GMP Protein, CF
Brand: rnd
SKU: 8879-GMP

Catalog # 8879-GMP

Datasheet / COA / SDS

Animal-Free

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Key Product Details

Source:	E. coli
Accession #:	Q9HBE4.3
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IL-21 GMP Protein, CF

Technical Data
Citations / Reviews / FAQs

Product Specifications

Source	E. coli-derived human IL-21 protein Gln32-Ser162, with a N-terminal Met Produced in an animal-free laboratory. Manufactured and tested under cGMP guidelines
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met-Gln-Asp-Arg-His-Met-Ile-Arg-Met-Arg
Predicted Molecular Mass	15 kDa
SDS-PAGE	16 kDa, reducing conditions
Activity	Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED₅₀ for this effect is ≤8 ng/mL.

Recombinant Human IL-21 GMP Protein, CF Scientific Data Examples

	GMP-grade Recombinant Human IL-21 (Catalog # 8879‑GMP) enhances IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED₅₀ for this effect is ≤8 ng/mL. Three independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of GMP IL-21.
	2 μg/lane of GMP-grade Recombinant Human IL-21 (Catalog # 8879-GMP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing R band at 16.4 kDa and NR band at 14.8 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8879-GMP

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100-200 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-21

Interleukin-21 (IL-21) is an approximately 14 kDa four-helix-bundle member of the family of cytokines that utilize the common gamma chain ( gamma _c) as a receptor subunit. gamma _c is also a subunit of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15 (1). IL-21 is produced by activated T follicular helper cells (Tfh), Th17 cells, and NKT cells (2-6). It exerts its biological effects through a heterodimeric receptor complex of gamma _c and the IL-21-specific IL-21 R (2, 7). Tfh-derived IL-21 plays an important role in the development of humoral immunity through its autocrine effects on the Tfh cell and paracrine effects on immunoglobulin affinity maturation, plasma cell differentiation, and B cell memory responses (4, 8, 9). It is also required for the migration of dendritic cells to draining lymph nodes (10). IL-21 regulates several aspects of T cell function. It co-stimulates the activation, proliferation, and survival of CD8⁺ T cells and NKT cells and promotes Th17 cell polarization (3, 5, 6, 11, 12). It blocks the generation of regulatory T cells and their suppressive effects on CD4⁺ T cells (13, 14). IL-21 R engagement enhances the cytolytic activity and IFN-gamma production of activated NK cells but limits the expansion of resting NK cells (15). In addition, IL-21 suppresses cutaneous hypersensitivity reactions by limiting allergen-specific IgE production and mast cell degranulation (16). Dysregulation of the IL-21/IL-21 R system contributes to the development of multiple immunological disorders (1, 17). The 133 amino acid (aa) mature human IL-21 shares 63% and 61% aa sequence identity with mouse and rat IL-21, respectively. Alternative splicing generates an additional isoform with a substitution of the C-terminal 16 amino acids (18).

Item	Value
References	Tangye, S.G. (2015) Curr. Opin. Immunol. 34:107. Parrish-Novak, et al. (2000) Nature 408:57. Coquet, J.M. et al. (2007) J. Immunol. 178:2827. Vogelzang, A. et al. (2008) Immunity 29:127. Korn, T. et al. (2007) Nature 448:484. Nurieva, R. et al. (2007) Nature 448:480. Asao, H. et al. (2001) J. Immunol. 167:1. Zotos, D. et al. (2010) J. Exp. Med. 207:365. Rankin, A.L. et al. (2011) J. Immunol. 186:667. Jin, H. et al. (2009) J. Clin. Invest. 119:47. Frohlich, A. et al. (2009) Science 324:1576. Yi, J.S., et al. (2009) Science 324:1572. Peluso, I. et al. (2007) J. Immunol. 178:732. Bucher, C. et al. (2009) Blood 114:5375. Kasaian, M.T. et al. (2002) Immunity 16:559. Tamagawa-Mineoka, R. et al. (2011) J. Invest. Dermatol. 131:1513. Ma, J. et al. (2011) Cytokine 56:133. Rahman, M. et al. (2007) FEBS Lett. 581:4001.
Long Name	Interleukin 21
Entrez Gene IDs	59067 (Human); 60505 (Mouse); 365769 (Rat); 442935 (Canine); 100344172 (Rabbit)
Alternate Names	CVID11, IL-21, IL-21Za11interleukin-21, IL21, Za11, interleukin 21, interleukin-21 isoform

Recombinant Human IL-21 GMP Protein, CFGMP

Order Information

R&D Systems Guarantee

Key Product Details

Product Specifications

Recombinant Human IL-21 GMP Protein, CF Scientific Data Examples

Formulation, Preparation and Storage

Carrier Free

Carrier Free

Reconstitution Calculator

Background: IL-21

Citations

Product Datasheets

Product Datasheets

COA

Certificate of Analysis

SDS

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