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Order Information
Key Product Details
Source: | E. coli |
Accession #: | Q9HBE4.3 |
Applications: | Bioactivity |
Product Specifications
Source |
E. coli-derived human IL-21 protein Gln32-Ser162, with a N-terminal Met Produced in an animal-free laboratory. Manufactured and tested under cGMP guidelines |
Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin level | <0.10 EU per 1 μg of the protein by the LAL method. |
N-terminal sequence Analysis |
Met-Gln-Asp-Arg-His-Met-Ile-Arg-Met-Arg |
Predicted Molecular Mass | 15 kDa |
SDS-PAGE | 16 kDa, reducing conditions |
Activity | Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is ≤8 ng/mL. |
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GMP-grade Recombinant Human IL-21 (Catalog # 8879‑GMP) enhances IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is ≤8 ng/mL. Three independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of GMP IL-21. |
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2 μg/lane of GMP-grade Recombinant Human IL-21 (Catalog # 8879-GMP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing R band at 16.4 kDa and NR band at 14.8 kDa, respectively. |
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier Free
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100-200 μg/mL in PBS. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: IL-21
Item | Value |
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References |
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Long Name | Interleukin 21 |
Entrez Gene IDs | 59067 (Human); 60505 (Mouse); 365769 (Rat); 442935 (Canine); 100344172 (Rabbit) |
Alternate Names | CVID11, IL-21, IL-21Za11interleukin-21, IL21, Za11, interleukin 21, interleukin-21 isoform |
Citations
Currently there are no citations for Recombinant Human IL-21 GMP Protein, CF.