Recombinant Human IL-2, Animal-Free Protein

Name: Recombinant Human IL-2, Animal-Free Protein
Brand: rnd
SKU: AFL202

Catalog # AFL202

Also available, GMP IL-2 Protein, Catalog # 202-GMP! Animal-free and manufactured under GMP guidelines for therapeutic manufacturing applications.

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Key Product Details

Source:	E. coli
Accession #:	P60568
Applications:	Bioactivity

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Product Specifications

Source	E. coli-derived human IL-2 protein Ala21-Thr153, with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory.
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	15 kDa
Activity	Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED 50 for this effect is 0.05-0.25 ng/mL. The specific activity of Recombinant Human IL-2 is approximately 2.1 x 10 4 IU/μg, which is calibrated against recombinant human IL-2 WHO International Standard (NIBSC code: 86/500).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AFL202

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile 100 mM Acetic Acid. Alternatively, reconstitute at 0.1 mg/mL in sterile deionized water and use within 24 hours, store at 2 to 8 degreesC.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 3 months, 2 to 8 degreesC under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Materials

Animal Free Proteins
Animal-free proteins are particularly important for researchers concerned with experimental variables caused by trace animal components or mammalian pathogens. Our products generated under animal-free conditions share the same biological activities as those produced using our standard laboratory techniques.

Animal Free Proteins

Background: IL-2

Interleukin-2 (IL-2) is a O-glycosylated, four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4 + and CD8 + T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1 - 3). Mature human IL-2 shares 56% and 66% amino acid sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity (4). The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (5 - 7). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4 + T cells but not activated CD4 + memory lymphocytes (8). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (9-11). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (12, 13).

Item	Value
References	Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109. Taniguchi, T. et al. (1983) Nature 302:305. Mosmann, T.R. et al. (1987) J. Immunol. 138:1813. Liparoto, S.F. et al. (2002) Biochemistry 41:2543. Wang, X. et al. (2005) Science 310:1159. Bodnar, A. et al. (2008) Immunol. Lett. 116:117. Jaleco, S. et al. (2003) J. Immunol. 171:61. Malek, T.R. (2003) J. Leukoc. Biol. 74:961. Laurence, A. et al. (2007) Immunity 26:371. Kryczek, I. et al. (2007) J. Immunol. 178:6730. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.
Long Name	Interleukin 2
Entrez Gene IDs	3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names	Aldesleukin, IL-2, IL-2lymphokine, IL2, T cell growth factor, T-cell growth factor, TCGF, interleukin 2, interleukin-2, involved in regulation of T-cell clonal expansion

Manufacturing Specifications

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

All molecular biology procedures use animal-free media and dedicated labware.
Dedicated fermentors are utilized in committed animal-free areas.

Purification

Protein purification columns are animal-free.
Bulk proteins are filtered using animal-free filters.
Purified proteins are stored in animal-free containers in a dedicated cold storage room.

Quality Assurance

Low Endotoxin Level.
No impairment of biological activity.
High quality product obtained under stringent conditions.
For ex vivo research or bioproduction, additional documentation can be provided.

Please read our complete Animal-Free Statement

Citations (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-2, Animal-Free Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

TA Olafsdotti et al. (2020), Eighty-eight variants highlight the role of T cell regulation and airway remodeling in asthma pathogenesis Nat Commun, 11(1):393.
PMID: 31959851
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Souheil-Antoine Younes (2016), IL-15 promotes activation and expansion of CD8+ T cells in HIV-1 infection J Clin Invest, 126(7):2745-2756.
PMID: 27322062

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