Recombinant Human IL-10 (aa 19-178) Protein

Name: Recombinant Human IL-10 (aa 19-178) Protein
Brand: rnd
SKU: 1064-IL

Catalog # 1064-IL

Citations (2)

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Key Product Details

Source:	E. coli
Accession #:	P22301
Structure / Form:	Noncovalently-linked homodimer
Applications:	Bioactivity

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Product Specifications

Source	E. coli-derived human IL-10 protein Ser19-Asn178, with an N-terminal Met
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	18.8 kDa
SDS-PAGE	18.4 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using MC/9‑2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED₅₀ for this effect is 0.15-0.75 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1064-IL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1064-IL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-10

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL‑10 family of alpha ‑helical cytokines that also includes IL‑19, IL‑20, IL‑22, IL‑24, and IL‑26/AK155 (1, 2). IL‑10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2 ‑ 5). Mature human IL‑10 shares 72% ‑ 86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL‑10. Whereas human IL‑10 is active on mouse cells, mouse IL‑10 does not act on human cells (6, 7). IL‑10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (6, 8, 9). The IL‑10 dimer binds to two IL‑10 R alpha /IL‑10 R1 chains, resulting in recruitment of two IL‑10 R beta /IL‑10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (10). IL‑10 R beta does not bind IL‑10 by itself but is required for signal transduction (1). IL‑10 R beta also associates with IL‑20 R alpha, IL‑22 R alpha, or IL‑28 R alpha to form the receptor complexes for IL‑22, IL‑26, IL‑28, and IL‑29 (11 ‑ 13). IL‑10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (14 ‑ 16). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

Item	Value
References	Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. Sabat, R. et al. (2010) Cytokine Growth Factor Rev. 21:331. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172. Hsu, D.-H. et al. (1990) Science 250:830. Windsor, W.T. et al. (1993) Biochemistry 32:8807. Syto, R. et al. (1998) Biochemistry 37:16943. Kotenko, S.V. et al. (1997) EMBO J. 16:5894. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725. Hor, S. et al. (2004) J. Biol. Chem. 279:33343. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269. Blackburn, S.D. and E.J. Wherry (2007)Trends Microbiol. 15:143.
Long Name	Interleukin 10
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Alternate Names	CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL-10, IL-10MGC126451, IL10, IL10A, TGIF, interleukin 10, interleukin-10

Citations (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-10 (aa 19-178) Protein include:

Species: Human
Sample Types: Serum
Applications: ELISA Standard

A Stumpf et al. (2019), Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum PLoS ONE, 14(12):e0225525.
PMID: 31790441
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Gruber SG et al. (2008), Differential signaling of cmvIL-10 through common variants of the IL-10 receptor 1. Eur. J. Immunol., 38(12):3365-75.
PMID: 19016528

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Product Datasheets

Download Product Datasheets: 1064-IL (with carrier)

Download Product Datasheets: 1064-IL/CF (carrier free)

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Recombinant Human IL-10 (aa 19-178) Protein

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Key Product Details

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Product Specifications

Formulation, Preparation and Storage

Carrier Free

1064-IL

Carrier

1064-IL/CF

Carrier Free

Reconstitution Calculator

Background: IL-10

Citations (2)

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