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Recombinant Human IgG1 Fc Alexa Fluor® 647 Protein

Bio-Techne includes R&D Systems | Catalog # AFR110

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AFR110-020

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide linked homodimer
Labeled with Alexa Fluor® 647
Excitation Wavelength: 650 nm
Emission Wavelength: 668 nm

Conjugate

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human IgG1 protein
IEGRMD Human IgG1
Pro100-Lys330
(Leu117Ala, Leu118Glu, Gly120Ala, Thr276Ala)
Accession # P01857.1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile

Predicted Molecular Mass

26.6 kDa

SDS-PAGE

30-40 kDa, under reducing conditions.

Activity

Measured by flow cytometry for its ability to bind anti-human IgG1 Fc Antibody conjugated beads. The concentration of Recombinant Human IgG1 Fc Alexa Fluor® 647 (Catalog # AFR110) that produces 50% of the binding response is 0.200-2.00 ng/mL.

Scientific Data Images for Recombinant Human IgG1 Fc Alexa Fluor® 647 Protein

Flow cytometry analysis for Recombinant Human IgG1 Fc Alexa Fluor® 647 staining on anti-human IgG1 Fc Antibody conjugated beads.

Streptavidin coated beads conjugated to biotinylated anti-human IgG1 Fc Antibody were stained with the indicated concentrations of Recombinant Human IgG1 Fc Alexa Fluor® 647 (Catalog # AFR110).

Recombinant Human IgG1 Fc Alexa Fluor® 647 Protein SDS-PAGE.

2 μg/lane of Recombinant Human IgG1 Fc Alexa Fluor® 647 Protein (Catalog # AFR110) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-35 kDa and 60-70 kDa, respectively.

Formulation, Preparation and Storage

AFR110
Formulation Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: IgG1

Human Fc is the crystallizable fragment in the constant region of the human immunoglobulin heavy chain. Members of the immunoglobulin superfamily (IgSF) consist of two heavy (H) and two light (L) chains, with each component containing one variable (V) and one or more constant (C) domains (1, 2). Produced by B lymphocytes, Ig gamma (IgG) is the predominant isotype found in the body and has the longest half-life of all immunoglobulin isotypes (3). Four IgG subclasses (IgG1-4) were identified based on structural, antigenic and functional differences in the constant region of the heavy chain. The constant tail of the antibody is where proteins involved in immune responses typically bind, most notably the first component of the complement pathway, C1q, and Fc receptors found on immune cells such as B lymphocytes. Affinities for C1q and Fc gamma receptor (Fc gammaR) differ among the IgG subclasses. Fc receptors mediate the recruitment of immune cells to a site of infection (4). Engineered crystallizable fragment (Fc) regions of antibody which assume a unique and unprecedented asymmetric structure within the homodimeric Fc polypeptide, enable completely selective binding to the complement component C1q and activation of complement via the classical pathway without any concomitant engagement of the Fc gammaR (5).

References

  1. Williams, A.F. and A.N. Barclay (1988) Annu. Rev. Immunol. 6:381.
  2. Harpaz, Y. and C. Chothia (1994) J. Mol. Biol. 238:528.
  3. Schroeder, H.W. and L. Cavacini (2010) J. Allergy Clin. Immunol. 125:S41.
  4. Duncan, A.R. and G. Winter (1998) Nature 332:738.
  5. Lee, C.H. et al. (2017) Nat. Immunol. 18:889.

Long Name

Immunoglobulin G1

Alternate Names

IgG1, Igh-4, RGD1359539, VH7183

UniProt

Additional IgG1 Products

Product Documents for Recombinant Human IgG1 Fc Alexa Fluor® 647 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IgG1 Fc Alexa Fluor® 647 Protein


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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