E. coli-derived human HPRT protein Ala2-Ala218 with an N-terminal Met and 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
27-29 kDa, under reducing conditions
Measured by its ability to produce GMP from guanine and phosphoribosyl pyrophosphate. The specific activity is >9000 pmol/min/μg, as measured under the described conditions.
Recombinant Human HPRT His-tag Protein, CF Scientific Data Examples
Recombinant Human HPRT His-tag Protein Enzyme Activity
Recombinant Human HPRT Hig-tag (Catalog # 10314-HP) is measured by its ability to produce GMP from guanine and phosphoribosyl pyrophosphate. The activity (orange) is over 2-fold higher than the competitor's HPRT (blue).
Recombinant Human HPRT His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Human HPRT His-tag (Catalog # 10314-HP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 27-29 kDa under reducing conditions.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and TCEP.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after opening.
Hypoxanthine-guanine phosphoribosyltransferase (HPRT), a member of the phosphoribosyltransferase family, is a magnesium-dependent, cytoplasmic, ubiquitously-expressed (1) salvage enzyme involved in the primary pathway utilized for purine synthesis (2, 3). It catalyzes the transfer of phosphoribose from phosphoribosyl pyrophosphate to hypoxanthine and guanine bases to form inosine and guanine, respectively. Human HPRT forms a homotetramer of identical subunits that form dimer pairs (4, 5). Each 49 kDa subunit is 217 amino acids and consists of a core domain containing the active site and a hood domain (4, 5). HPRT deficiency in the salvage pathway leads to overproduction of purine, indicating it may play a role in regulation of purine production (6). Deficiency from multiple characterized single point mutations (7,8) causes hyperuricemia resulting in gout-like symptoms of Kelley-Seegmiller syndrome or if completely lacking in HPRT, a severe X-linked hereditary disorder, Lesch-Nyhan Disease, that is also characterized to cause significant neurological impairment (3, 6) by affecting multiple signaling pathways (9) and through dysregulation of cellular functions including proliferation, RNA metabolism, DNA replication and protein synthesis (3, 10). Given its ability to modulate cellular functions through purine regulation, HPRT is implicated to play a role in cancer. Significant correlation between HPRT mutations and increased cancer risk have been reported (11, 12). HPRT has been found surface-expressed in cancer (13) and thus has been proposed as a potential marker and therapeutic target (12).
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Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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