Recombinant Human His6-ATG3 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # E2-670
Discontinued Product
E2-670 has been discontinued.
View all ATG3 products.
Key Product Details
Product Specifications
Source
E. coli-derived human ATG3 protein
Contains a C-terminal 6-His tag
Contains a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
37 kDa
Activity
Recombinant Human His6-Apg3p/ATG3/Apg3L is a member of the Apg-conjugating (E2) enzyme family that receives Apg from a Apg-activating (E1) enzyme and subsequently interacts with a Apg ligase (E3) to conjugate Apg to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Apg3p/ATG3/Apg3L concentration of 0.1-1 μM.
Formulation, Preparation and Storage
E2-670
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: ATG3
Autophagy-related 3 (ATG3), also known as Apg3L and Apg3p, functions as a regulatory component of autophagosome biogenesis necessary for autophagy. It is a 314 amino acid (aa) protein with a predicted molecular weight of 36 kDa. ATG3 exhibits 98% aa sequence identity with both its mouse and rat orthologs. It is a Ubiquitin-conjugating (E2)-like enzyme that works in concert with Atg12-Atg5, a Ubiquitin Ligase (E3)-like enzyme conjugate, to catalyze the transfer of Atg8 (LC3, GATE-16, and GABARAP in mammals) to phosphatidylethanolamine (PE) in the developing autophagosome membrane (1-4). This lipidation of Atg8 proteins is necessary for autophagosome maturation (5).
References
- Ichimura, Y. et al. (2000) Nature 408:488.
- Ichimura, T. et al. (2004) J. Biol. Chem. 279:40584.
- Yamaguchi, M. et al. (2010) J. Biol. Chem. 285:29599.
- Otomo, C. et al. (2013) Nat. Struct. Mol. Biol. 20:59.
- Shpilka, T. et al. (2012) Genome Biol. 12:226.
Long Name
Autophagy Related 3 Homolog
Alternate Names
APG3L, Apg3p, PC3-96
Gene Symbol
ATG3
UniProt
Additional ATG3 Products
Product Documents for Recombinant Human His6-ATG3 Protein, CF
Product Specific Notices for Recombinant Human His6-ATG3 Protein, CF
For research use only
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