Recombinant Human HIN-1/SCGB3A1 Protein, CF

Catalog # 2790-HN | R&D Systems, Inc. a Bio-Techne Brand
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2790-HN-050
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Key Product Details

Accession #

Q96QR1

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human HIN-1/SCGB3A1 protein
Phe21-Gly104

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe21

Predicted Molecular Mass

8.2 kDa

SDS-PAGE

6 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cells.
The ED50 for this effect is 0.15-0.75 μg/mL.

Formulation, Preparation and Storage

2790-HN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIN-1/SCGB3A1

High in Normal‑1 (HIN‑1), also known as Uteroglobin related protein 2 (UGRP2), is an 8 kDa secreted protein of the secretoglobin superfamily (SCGB3A1) (1). HIN‑1 is expressed in bronchial epithelial and secretory Clara cells, mammary epithelial cells (particularly during pregnancy), salivary glands, and the prostate (2‑5). It is up‑regulated in Clara cells by IL‑4, IL‑13, Oncostatin M, EGF, and TGF‑ alpha and down‑regulated by IFN‑ alpha, beta, and gamma (6‑9). It is secreted into bronchial lavage fluid, saliva, and plasma, and may form disulfide‑linked dimers (10). HIN‑1 binds to the macrophage scavenger receptor MARCO, and to the surface of mammary and bronchial epithelial cells (11, 12). HIN‑1 promotes apoptosis and inhibits the proliferation, migration, and invasion of breast cancer cells (12). The down‑regulation of HIN‑1 expression in many breast, lung, and prostate cancers correlates with hypermethylation of its promoter (4, 10, 13, 14). Mature human HIN‑1 shares 58% and 62% aa sequence identity with mouse and rat HIN‑1, respectively (4).

References

  1. Mukherjee, A.B. et al. (2007) Endocr. Rev. 28:707.
  2. Porter, D. et al. (2002) Mech. Dev. 114:201.
  3. Reynolds, S.D. et al. (2002) Am. J. Respir. Crit. Care Med. 166:1498.
  4. Krop, I. et al. (2001) Proc. Natl. Acad. Sci. 98:9796.
  5. Niimi, T. et al. (2002) Cytogenet. Genome Res. 97:120.
  6. Yamada, A. et al. (2005) J. Immunol. 175:5708.
  7. Tomita, T. et al. (2009) Am. J. Respir. Cell Mol. Biol. 40:620.
  8. Yamada, A. and S. Kimura (2005) FEBS Lett. 579:2221.
  9. Yamada, A. et al. (2009) Biochem. Biophys. Res. Commun. 379:964.
  10. Krop, I. et al. (2004) Mol. Cancer Res. 2:489.
  11. Bin, L.-H. et al. (2003) J. Immunol. 171:924.
  12. Krop, I. et al. (2005) Cancer Res. 65:9659.
  13. Krop, I. et al. (2003) Cancer Res. 63:2024.
  14. Shigematsu, H. et al. (2005) Int. J. Cancer 113:600.

Long Name

High in Normal 1

Alternate Names

HIN1, PnSP-2, SCGB3A1, UGRP2

Entrez Gene IDs

92304 (Human); 73945 (Mouse)

Gene Symbol

SCGB3A1

UniProt

Q96QR1

Product Documents for Recombinant Human HIN-1/SCGB3A1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human HIN-1/SCGB3A1 Protein, CF

For research use only

Reconstitution Calculator

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