Source	Mouse myeloma cell line, NS0-derived human HAI-1 protein Pro37-Glu449, with a C-terminal 10-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Pro37
Predicted Molecular Mass	47 kDa
SDS-PAGE	58 kDa, reducing conditions
Activity	Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH₂ (Catalog # ES002). The IC₅₀ is <2 nM, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1048-PI

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile, deionized water. Reconstitution Buffer Available: Size / Price Qty Loading... RB07:
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HAI-1

HAI-1 is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matriptase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate 13-acetate or IL-1 beta. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).

Item	Value
References	Denda, K. et al. (2002) J. Biol. Chem. 277:14053. Shimomura, T. et al. (1997) J. Biol. Chem. 272:6370. Kataoka, H. et al. (2000) J. Biol. Chem. 275:40453.
Long Name	HGF Activator Inhibitor Type 1
Entrez Gene IDs	6692 (Human); 20732 (Mouse)
Alternate Names	HAI, HAI-1, HAI1, Hepatocyte growth factor activator inhibitor type 1, MANSC2, SPINT1, hepatocyte growth factor activator inhibitor 1, kunitz-type protease inhibitor 1, serine peptidase inhibitor, Kunitz type 1, serine protease inhibitor, Kunitz type 1

Citations for Recombinant Human HAI-1 Protein, CF (6)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human HAI-1 Protein, CF include:

Sample Types: Protein
Applications: Bioassay

R Szabo et al. (2018), Loss of HAI-2 in mice with decreased prostasin activity leads to an early-onset intestinal failure resembling congenital tufting enteropathy PLoS ONE, 13(4):e0194660.
PMID: 29617460
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

AS Murray et al. (2017), Phosphorylation of the type II transmembrane serine protease, TMPRSS13 in Hepatocyte Growth Factor Activator Inhibitor-1 and 2-mediated cell surface localization J. Biol. Chem., 0(0).
PMID: 28710277
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay

Sales KU et al. (2010), Matriptase initiates activation of epidermal pro-kallikrein and disease onset in a mouse model of Netherton syndrome. Nat. Genet., 42(8):676-83.
PMID: 20657595
Species: N/A
Sample Types: N/A
Applications: Degradation

Szabo R et al. (2008), Potent Inhibition and Global Co-localization Implicate the Transmembrane Kunitz-type Serine Protease Inhibitor Hepatocyte Growth Factor Activator Inhibitor-2 in the Regulation of Epithelial Matriptase Activity. J. Biol. Chem., 283(43):29495-504.
PMID: 18713750
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Butler GS et al. (2008), Pharmacoproteomics of a metalloproteinase hydroxamate inhibitor in breast cancer cells: dynamics of membrane type 1 matrix metalloproteinase-mediated membrane protein shedding. Mol. Cell. Biol., 28(15):4896-914.
PMID: 18505826
Species: N/A
Sample Types: Protein
Applications: Enzyme Assay

Cortesio CL et al. (2006), Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5. Arch. Biochem. Biophys., 449(1):164-70.
PMID: 16554018

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Recombinant Human HAI-1 Protein, CF

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