Is there a mass to unit conversion factor for Recombinant Human GM-CSF (CHO-expressed) Protein (Catalog # 7954-GM & 7954-GM/CF)?

Name: Recombinant Human GM-CSF (CHO-expressed) Protein
Brand: R&D Systems
SKU: 7954-GM
Rating: 5 (7 reviews)

We have not developed a specific mass to unit conversion factor for Recombinant Human GM-CSF CHO-expressed Protein (Catalog # 7954-GM & 7954-GM/CF). That said, because Catalog # 7954-GM and Recombinant Human GM-CSF E. coli-expressed Protein (Catalog # 215-GM) share an ED50 range for our routine QC assay (Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 6-30 pg/mL), it is possible to use the mass to unit conversion factor for Catalog # 215-GM as a starting point for Catalog # 7954-GM. Catalog # 215-GM has been evaluated against human GM-CSF WHO International Standard (NIBSC code: 88/646). The specific activity of Recombinant Human GM-CSF (Catalog # 215-GM) is approximately 1.5 x 104 IU/μg, which is calibrated against human GM-CSF WHO International Standard (NIBSC code: 88/646). Since units of activity can vary per assay, we would recommend that end-users develop a conversion factor for their individual application.

Recombinant Human GM-CSF (CHO-expressed) Protein

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 7954-GM

Citations (2)

(7)

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Key Product Details

Source:	CHO
Accession #:	P04141
Applications:	Bioactivity

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Product Specifications for Recombinant Human GM-CSF (CHO-expressed) Protein

Source	Chinese Hamster Ovary cell line, CHO-derived human GM-CSF protein Ala18-Glu144
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala18
Predicted Molecular Mass	14.5 kDa
SDS-PAGE	20-35 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 6-30 pg/mL.

Scientific Data Examples for Recombinant Human GM-CSF (CHO-expressed) Protein

	Recombinant Human GM-CSF (CHO-expressed) Protein Bioactivity Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 6-30 pg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

7954-GM

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7954-GM/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GM-CSF

GM‑CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte‑macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM‑CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM‑CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM‑CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3‑5). It shows clinical effectiveness in ameliorating chemotherapy‑induced neutropenia, and GM‑CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM‑CSF, similar to IL‑3 and IL‑5, is a cytokine with a core of four bundled alpha ‑helices (8‑12). Mature human GM‑CSF shares 63%‑70% amino acid sequence identity with canine, feline, porcine, and rat GM‑CSF and 54% with mouse GM‑CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM‑CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high‑affinity receptors for IL‑3 and IL‑5 (13, 14). In addition, GM‑CSF binds a naturally occurring soluble form of GM‑CSF R alpha (15). Human GM‑CSF is active on canine and feline cells but not on murine cells (16‑18).

Item	Value
References	Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163. Cao, Y. (2007) J. Clin. Invest. 117:2362. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405. Heuser, M. et al. (2007) Semin. Hematol. 44:148. Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321. Kaushansky, K. et al. (1992) Biochemistry 31:1881. Diederichs, K. et al. (1991) Science 254:1779. Cantrell, M.A. et al. (1985) Proc. Natl. Acad. Sci. 82:6250. Lee, F. et al. (1985) Proc. Natl. Acad. Sci. 82:4360. Wong, G.G. et al. (1985) Science 228:810. Onetto-Pothier, N. et al. (1990) Blood 75:59. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483. Hogge, G.S. et al. (1990) Cancer Gene Ther. 6:26. Sprague, W.S. et al. (2005) J. Comp. Pathol. 133:136. Shanafelt, A.B. et al. (1991) J. Biol. Chem. 266:13804.
Long Name	Granulocyte Macrophage Growth Factor
Entrez Gene IDs	1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
Alternate Names	CSF, CSF-2, CSF2, Colony-stimulating factor, GM-CSF, GMCSF, GMCSFgranulocyte-macrophage colony-stimulating factor, MGC131935, MGC138897, Molgramostim, Sargramostim, colony stimulating factor 2 (granulocyte-macrophage), granulocyte-macrophage colony stimulating factor, molgramostin

Citations for Recombinant Human GM-CSF (CHO-expressed) Protein (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human GM-CSF (CHO-expressed) Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Zhu et al. (2022), Extracellular Vesicle-Derived circITGB1 Regulates Dendritic Cell Maturation and Cardiac Inflammation via miR-342-3p/NFAM1 Oxidative Medicine and Cellular Longevity, 2022(0):8392313.
PMID: 35615580
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

M Medelin et al. (2018), Bridging pro-inflammatory signals, synaptic transmission and protection in spinal explants in vitro Mol Brain, 11(1):3.
PMID: 29334986

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Customer Reviews for Recombinant Human GM-CSF (CHO-expressed) Protein (7)

4.9 out of 5

7 customer ratings

5 stars

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Recombinant Human GM-CSF (CHO-expressed) Protein

Name: Anonymous

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 12/18/2020
Recombinant Human GM-CSF (CHO-expressed) Protein

Name: Jayalakshmi Miriyala

Application: Stem/Immune cell maintenance or differentiation

Reason for Rating: worked very well for the differentiation experiments

Verified Customer | Posted 10/01/2020
Recombinant Human GM-CSF (CHO-expressed) Protein

Name: Robert Berahovich

Application: In vitro bioactivity in cell culture

Reason for Rating: Excellent for differentiation of human monocytes into dendritic cells (along with IL-4). Cells were cultured for 6 days with GM-CSF at 67 ng/ml.

Verified Customer | Posted 09/29/2020

Flow cytometric of human monocytes (left) before and after differentiation into dendritic cells (right). CD1a-CD14+ monocytes are in the upper left quadrant, whereas CD1a+CD14- dendritic cells are in the lower right quadrant.
Recombinant Human GM-CSF (CHO-expressed) Protein

Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 09/16/2019
Recombinant Human GM-CSF (CHO-expressed) Protein

Name: Mahesh Raundhal

Application: Cell differentiation

Reason for Rating: Excellent at inducing CD14+ monocyte differentiation into CD1a+ DCs.

Verified Customer | Posted 11/19/2017

Enriched CD14+ monocytes were cultured with 500 IU/mL IL-4 and 1000 IU/mL GM-CSF for 6 days.
Recombinant Human GM-CSF (CHO-expressed) Protein

Name: Anonymous

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 05/16/2017
Recombinant Human GM-CSF (CHO-expressed) Protein

Name: jacqueline lee

Application: In vitro bioactivity in cell culture

Reason for Rating: 100ng/ml of this cytokine +IL-4 helped to differentiate CD14+ monocytes into CD14 negative dendritic cells in 5 days.

Verified Customer | Posted 08/09/2016

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FAQs for Recombinant Human GM-CSF (CHO-expressed) Protein

Is there a mass to unit conversion factor for Recombinant Human GM-CSF (CHO-expressed) Protein (Catalog # 7954-GM & 7954-GM/CF)?

We have not developed a specific mass to unit conversion factor for Recombinant Human GM-CSF CHO-expressed Protein (Catalog # 7954-GM & 7954-GM/CF). That said, because Catalog # 7954-GM and Recombinant Human GM-CSF E. coli-expressed Protein (Catalog # 215-GM) share an ED₅₀ range for our routine QC assay (Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 6-30 pg/mL), it is possible to use the mass to unit conversion factor for Catalog # 215-GM as a starting point for Catalog # 7954-GM. Catalog # 215-GM has been evaluated against human GM-CSF WHO International Standard (NIBSC code: 88/646). The specific activity of Recombinant Human GM-CSF (Catalog # 215-GM) is approximately 1.5 x 10⁴ IU/μg, which is calibrated against human GM-CSF WHO International Standard (NIBSC code: 88/646). Since units of activity can vary per assay, we would recommend that end-users develop a conversion factor for their individual application.

Recombinant Human GM-CSF (CHO-expressed) Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications for Recombinant Human GM-CSF (CHO-expressed) Protein

Scientific Data Examples for Recombinant Human GM-CSF (CHO-expressed) Protein

Formulation, Preparation and Storage

Carrier Free

7954-GM

Carrier

7954-GM/CF

Carrier Free

Reconstitution Calculator

Background: GM-CSF

Citations for Recombinant Human GM-CSF (CHO-expressed) Protein (2)

Customer Reviews for Recombinant Human GM-CSF (CHO-expressed) Protein (7)

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Recombinant Human GM-CSF (CHO-expressed) Protein

Recombinant Human GM-CSF (CHO-expressed) Protein

Recombinant Human GM-CSF (CHO-expressed) Protein

Recombinant Human GM-CSF (CHO-expressed) Protein

Recombinant Human GM-CSF (CHO-expressed) Protein

Recombinant Human GM-CSF (CHO-expressed) Protein

Recombinant Human GM-CSF (CHO-expressed) Protein

FAQs for Recombinant Human GM-CSF (CHO-expressed) Protein

Product Documents for Recombinant Human GM-CSF (CHO-expressed) Protein

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