Recombinant Human GM-CSF (CHO-expressed) Protein
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Source: | CHO |
Accession #: | P04141 |
Applications: | Bioactivity |
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Citations for Recombinant Human GM-CSF (CHO-expressed) Protein (2)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human GM-CSF (CHO-expressed) Protein include:
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Species: Human
Sample Types: Whole Cells
Applications: BioassayJ Zhu et al. (2022), Extracellular Vesicle-Derived circITGB1 Regulates Dendritic Cell Maturation and Cardiac Inflammation via miR-342-3p/NFAM1 Oxidative Medicine and Cellular Longevity, 2022(0):8392313.
PMID: 35615580 -
Species: Mouse
Sample Types: Whole Tissue
Applications: BioassayM Medelin et al. (2018), Bridging pro-inflammatory signals, synaptic transmission and protection in spinal explants in vitro Mol Brain, 11(1):3.
PMID: 29334986
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Customer Reviews for Recombinant Human GM-CSF (CHO-expressed) Protein (7)
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 12/18/2020
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Application: Stem/Immune cell maintenance or differentiationReason for Rating: worked very well for the differentiation experimentsVerified Customer | Posted 10/01/2020
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Application: In vitro bioactivity in cell cultureReason for Rating: Excellent for differentiation of human monocytes into dendritic cells (along with IL-4). Cells were cultured for 6 days with GM-CSF at 67 ng/ml.Verified Customer | Posted 09/29/2020Flow cytometric of human monocytes (left) before and after differentiation into dendritic cells (right). CD1a-CD14+ monocytes are in the upper left quadrant, whereas CD1a+CD14- dendritic cells are in the lower right quadrant.
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 09/16/2019
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Application: Cell differentiationReason for Rating: Excellent at inducing CD14+ monocyte differentiation into CD1a+ DCs.Verified Customer | Posted 11/19/2017Enriched CD14+ monocytes were cultured with 500 IU/mL IL-4 and 1000 IU/mL GM-CSF for 6 days.
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 05/16/2017
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Application: In vitro bioactivity in cell cultureReason for Rating: 100ng/ml of this cytokine +IL-4 helped to differentiate CD14+ monocytes into CD14 negative dendritic cells in 5 days.Verified Customer | Posted 08/09/2016
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FAQs for Recombinant Human GM-CSF (CHO-expressed) Protein
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Is there a mass to unit conversion factor for Recombinant Human GM-CSF (CHO-expressed) Protein (Catalog # 7954-GM & 7954-GM/CF)?
We have not developed a specific mass to unit conversion factor for Recombinant Human GM-CSF CHO-expressed Protein (Catalog # 7954-GM & 7954-GM/CF). That said, because Catalog # 7954-GM and Recombinant Human GM-CSF E. coli-expressed Protein (Catalog # 215-GM) share an ED50 range for our routine QC assay (Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 6-30 pg/mL), it is possible to use the mass to unit conversion factor for Catalog # 215-GM as a starting point for Catalog # 7954-GM. Catalog # 215-GM has been evaluated against human GM-CSF WHO International Standard (NIBSC code: 88/646). The specific activity of Recombinant Human GM-CSF (Catalog # 215-GM) is approximately 1.5 x 104 IU/μg, which is calibrated against human GM-CSF WHO International Standard (NIBSC code: 88/646). Since units of activity can vary per assay, we would recommend that end-users develop a conversion factor for their individual application.