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Recombinant Human GM-CSF (CHO-expressed) Protein

R&D Systems, Inc. a Bio-Techne Brand

Recombinant Human GM-CSF (CHO-expressed) Protein Bioactivity
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With Carrier
7954-GM-010

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Carrier Free
7954-GM-100/CF
7954-GM-050/CF
7954-GM-010/CF
7954-GM-020/CF
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Key Product Details

Source: CHO
Accession #: P04141
Applications: Bioactivity

Citations for Recombinant Human GM-CSF (CHO-expressed) Protein (2)

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Customer Reviews for Recombinant Human GM-CSF (CHO-expressed) Protein (7)

4.9 out of 5
7 customer ratings
5 stars
86%
4 stars
14%
3 stars
0%
2 stars
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1 star
0%

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  • Recombinant Human GM-CSF (CHO-expressed) Protein
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 12/18/2020
    Recombinant Human GM-CSF (CHO-expressed) Protein Binding assay/Protein-protein interaction 7954-GM
  • Recombinant Human GM-CSF (CHO-expressed) Protein
    Name: Jayalakshmi Miriyala
    Application: Stem/Immune cell maintenance or differentiation
    Reason for Rating: worked very well for the differentiation experiments
    Verified Customer | Posted 10/01/2020
  • Recombinant Human GM-CSF (CHO-expressed) Protein
    Name: Robert Berahovich
    Application: In vitro bioactivity in cell culture
    Reason for Rating: Excellent for differentiation of human monocytes into dendritic cells (along with IL-4). Cells were cultured for 6 days with GM-CSF at 67 ng/ml.
    Verified Customer | Posted 09/29/2020
    Flow cytometric of human monocytes (left) before and after differentiation into dendritic cells (right). CD1a-CD14+ monocytes are in the upper left quadrant, whereas CD1a+CD14- dendritic cells are in the lower right quadrant.
    Recombinant Human GM-CSF (CHO-expressed) Protein In vitro bioactivity in cell culture 7954-GM
  • Recombinant Human GM-CSF (CHO-expressed) Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 09/16/2019
    Recombinant Human GM-CSF (CHO-expressed) Protein In vitro bioactivity in cell culture 7954-GM
  • Recombinant Human GM-CSF (CHO-expressed) Protein
    Name: Mahesh Raundhal
    Application: Cell differentiation
    Reason for Rating: Excellent at inducing CD14+ monocyte differentiation into CD1a+ DCs.
    Verified Customer | Posted 11/19/2017
    Enriched CD14+ monocytes were cultured with 500 IU/mL IL-4 and 1000 IU/mL GM-CSF for 6 days.
    Recombinant Human GM-CSF (CHO-expressed) Protein Other 7954-GM
  • Recombinant Human GM-CSF (CHO-expressed) Protein
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 05/16/2017
  • Recombinant Human GM-CSF (CHO-expressed) Protein
    Name: jacqueline lee
    Application: In vitro bioactivity in cell culture
    Reason for Rating: 100ng/ml of this cytokine +IL-4 helped to differentiate CD14+ monocytes into CD14 negative dendritic cells in 5 days.
    Verified Customer | Posted 08/09/2016
    Recombinant Human GM-CSF (CHO-expressed) Protein In vitro bioactivity in cell culture 7954-GM

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FAQs for Recombinant Human GM-CSF (CHO-expressed) Protein

  • Is there a mass to unit conversion factor for Recombinant Human GM-CSF (CHO-expressed) Protein (Catalog # 7954-GM & 7954-GM/CF)?

    We have not developed a specific mass to unit conversion factor for Recombinant Human GM-CSF CHO-expressed Protein (Catalog # 7954-GM & 7954-GM/CF).  That said, because Catalog # 7954-GM and Recombinant Human GM-CSF E. coli-expressed Protein (Catalog # 215-GM) share an ED50 range for our routine QC assay (Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 6-30 pg/mL), it is possible to use the mass to unit conversion factor for Catalog # 215-GM as a starting point for Catalog # 7954-GM.  Catalog # 215-GM has been evaluated against human GM-CSF WHO International Standard (NIBSC code: 88/646).  The specific activity of Recombinant Human GM-CSF (Catalog # 215-GM) is approximately 1.5 x 104 IU/μg, which is calibrated against human GM-CSF WHO International Standard (NIBSC code: 88/646).  Since units of activity can vary per assay, we would recommend that end-users develop a conversion factor for their individual application.

Product Documents for Recombinant Human GM-CSF (CHO-expressed) Protein

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