Chinese Hamster Ovary cell line, CHO-derived human gamma-Glutamyltransferase 1/GGT1 protein Pro27-Tyr569 with a C-terminal 6-His
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Pro27 & Thr381
Predicted Molecular Mass
39 kDa (heavy chain) & 21 kDa (light chain)
56-69 kDa & 22-26 kDa, under reducing conditions
Measured by its ability to hydrolyze glutathione to Glu and Cys-Gly. The specific activity is >1300 pmol/min/μg, as measured under the described conditions.
Recombinant Human GGT1 His-tag Protein, CF Scientific Data Examples
Recombinant Human GGT1 His-tag Protein Enzyme Activiy.
Recombinant Human GGT1 His-tag (Catalog # 10977-GT) is measured by its ability to hydrolyze glutathione to Glu and Cys-Gly.
Recombinant Human GGT1 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human GGT1 His-tag Protein (Catalog # 10977-GT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 56-69 kDa and 22-26 kDa, under reducing conditions.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after opening.
Background: gamma-Glutamyltransferase 1/GGT1
Gamma-glutamyl transferase 1 (GGT1), also known as Gamma-glutamyl transpeptidase 1, is a ubiquitous enzyme that cleaves the gamma-glutamyl bond substrates where the glutamate moiety is available. This makes GGT1 crucial for metabolism of glutathione, the most important cellular antioxidant in humans and for its role in leukotriene biosynthesis, required for lipid metabolism. Although GGT1 comes from a 7 member multigene family, only GGT1 and GGT5 have enzymatic activity (1, 2). GGT5 has 40% amino acid homology with GGT1 and only 4% of the activity (2, 3) making GGT1 the primary contributor to enzymatic activity in the cells. GGT1 is synthesized as a single 569 amino acid polypeptide that is autocleaved by threonine 381 into a mature, activated, heterodimeric glycoprotein composed of a large subunit with an N-terminal hydrophobic transmembrane domain that anchors the enzyme to the membrane and a small catalytic subunit containing the catalytic nucleophile threonine 381 (4, 5). The human GGT1 active site is located in a deep channel but remains in an open configuration (4). It has a unique buried chloride ion that is integral to the protein structure (4). GGT1 contributes to cysteine homeostasis, intracellular redox, and inflammation (2, 6-8) and has been implicated to play a role in Parkinson and cardiovascular disease (5, 9), is a diagnostic marker of several diseases including liver and pancreatic cancer and is induced in several cancers to contribute to resistance to chemotherapeutic agents (7, 10-14).
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West, M.B. et al. (2013) J Biol. Chem. 288:31902.
Ndrepepa, G. et al. (2018) Clin. Chim. Acta. 476:130.
Lieberman, M.W. et al. (1996) Proc. Natl. Acad. Sci. 93: 7923.
Hanigan, M.H. (2014) Adv. Cancer Res. 122:103.
Corti, A. et al. (2020) Free Radic. Biol. Med.160:807.
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Lyu, S.C. et al. (2021) Cancer Manag. Res. 13:4887.
Carr, B.I. et al. (2021) J. Transl. Sci. 7:10.15761.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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