Source	Human embryonic kidney cell, HEK293-derived human Galectin-3C protein Gly108-Ile250
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Gly108
Predicted Molecular Mass	16 kDa
SDS-PAGE	14-18 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human Integrin alpha 5 beta 1 Recombinant Human Integrin alpha 5 beta 1 (Catalog # 3230-A5) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human Galectin-3C that produces 50% of the optimal binding response is 1-6 μg/mL.

Recombinant Human Galectin-3C Protein, CF Scientific Data Examples

	Recombinant Human Galectin-3C Protein Binding Activity When Recombinant Human Integrin a5 beta 1 (Catalog # 3230-A5) is coated at 1 µg/mL (100 µL/well), Recombinant Human Galectin-3C (Catalog # 10110-GA) binds with an ED₅₀ of 1-6 µg/mL
	Recombinant Human Galectin-3C Protein SDS-PAGE 2 μg/lane of Recombinant Human Galectin‑3C was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 14-18 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10110-GA

Formulation	Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, TCEP, PEG and Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in water.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Galectin-3C

Human Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a nonlectin domain (1, 2). The truncated form, Galectin-3C, which consists of the carboxy-terminal amino acid residues of Galectin-3 and lacks the N-terminal domain, has been shown to inhibit tumor growth and metastasis (3, 4). Within this region, human Galectin-3C shares 87% and 83% amino acid (aa) sequence identity with mouse and rat Galectin-3C, respectively. Galectin-3C has been found to interact with Integrin beta 1 in Hela cell lateral mobility assays (5). It can also be found endogenously attached to cell surface of neutrophils (6). Galectin-3C has been shown to enhance the activity of cancer therapy drugs as well as inhibiting tubule formation during angiogenesis (3).

Item	Value
References	Robertson, M.W. et al. (1990) Biochemistry 29:8093. Elola, M.T. et al. (2007) Cell. Mol. Life Sci. 64:1679. Mirandola, L. et al. (2011) PloS One 6:e21811. John, C.M. et al. (2003) Clin. Cancer Res. 9:2374. Yang, E.H. et al. (2017) PLoS ONE 12:e0184378. Sundqvist, M. et al. (2018) J. Leukoc. Biol. 103:341.
Long Name	Galectin-3, C-Terminus
Entrez Gene IDs	3958 (Human); 16854 (Mouse)
Alternate Names	Galectin-3C

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Recombinant Human Galectin-3C Protein, CF

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