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Recombinant Human G6b Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 5499-G6

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5499-G6-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human G6b protein
Human G6b
(Asn18-Gln142)
Accession # O95866
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn18

Predicted Molecular Mass

40.0 kDa (monomer)

SDS-PAGE

45-55 kDa under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When 1 μg/mL of rhG6b/Fc Chimera is captured by a goat anti-human IgG Fc Chimera coated plate, the concentration of biotinylated heparin that produces 50% of the optimal binding response is found to be approximately 10-40 ng/mL.

Formulation, Preparation and Storage

5499-G6
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: G6b

G6b is a type I transmembrane glycoprotein in the immunoglobulin superfamily that is encoded within the human class III major histocompatibility complex (1-3). It is expressed mainly on platelets, and has also been detected on megakaryocytes and the human cell line K562 (2-5). Crosslinking of G6b inhibits agonist-stimulated platelet aggregation and activation (5). The 241 amino acid (aa) human G6b isotype B (G6b-B) contains a 17 aa signal sequence, a 125 aa extracellular domain with one V-type Ig-like domain and one N-linked glycosylation site, a 21 aa transmembrane sequence and a 78 aa cytoplasmic sequence with two ITIM inhibitory motifs and a SH3 binding site (1, 2). Phosphorylation at Y211 within the first ITIM is required for interaction with SHP adaptor/tyrosine phosphatase proteins (2, 3). Of several described isoforms, all are identical within aa 22-136, which includes most of the extracellular domain. One contains an alternate signal sequence, while at least two lack a transmembrane sequence and are secreted (2). At least two others, including G6b-A, express alternate C-termini that lack the ITIM motifs. Of the known isoforms, G6b-A and G6b-B predominate in platelets. It is possible that G6b-A may be paired with and control inhibition by G6b-B (4). Heparin interacts with the G6b extracellular domain with high-affinity and may contribute to G6b activity or adhesion to extracellular matrix (6).

References

  1. Ribas, G. et al. (1999) J. Immunol. 163:278.
  2. DeVet, E. C. J. M. et al. (2001) J. Biol. Chem. 276:42070.
  3. Senis, Y. A. et al. (2007) Mol. Cell. Proteomics 6:548.
  4. Macaulay, I. C. et al. (2007) Blood 109:3260.
  5. Newland, S. A. et al. (2007) Blood 109:4806.
  6. DeVet, E. C. J. M. et al. (2005) FEBS Lett. 579:2355.

Long Name

Glycoprotein VI [Platelet]

Alternate Names

C6orf25, NG31

Entrez Gene IDs

80739 (Human); 106722 (Mouse); 406865 (Rat)

Gene Symbol

MPIG6B

UniProt

Additional G6b Products

Product Documents for Recombinant Human G6b Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human G6b Fc Chimera Protein, CF

For research use only

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