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Recombinant Human FGFR3 alpha (IIIb) His-tag Protein, CF

Catalog # 11136-FR | R&D Systems, Inc. a Bio-Techne Brand
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11136-FR-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human FGFR3 protein
Glu23-Gly377, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu23

Predicted Molecular Mass

39 kDa

SDS-PAGE

60-66 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human FGFR3 alpha (IIIb) His-tag (Catalog # 11136-FR) binds to Recombinant Human FGF acidic/FGF1 (aa 16-155) Protein (Catalog # 232-FA) with an ED50 of 0.700-7.00 μg/mL.

Scientific Data Images for Recombinant Human FGFR3 alpha (IIIb) His-tag Protein, CF

Recombinant Human FGFR3 alpha (IIIb) His-tag Protein Binding Activity.

Measured by its binding ability in a functional ELISA. Recombinant Human FGFR3 alpha (IIIb) His-tag Protein (Catalog # 11136-FR) binds to Recombinant Human FGF acidic/FGF1 (aa 16-155) Protein (232-FA) with an ED50 of 0.700-7.00 μg/mL.

Recombinant Human FGFR3 alpha (IIIb) His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human FGFR3 alpha (IIIb) His-tag Protein (Catalog # 11136-FR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-66 kDa.

Formulation, Preparation and Storage

11136-FR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR3

Fibroblast growth factor receptor 1 (FGFR1) belongs to a family of type I transmembrane tyrosine kinases which mediate the biological functions of FGFs that are involved in a multitude of physiological and pathological cellular processes (1). The FGFR family is comprised of 4 structurally conserved members (FGFR1-4) all possessing and extracellular domain (ECD) with three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain (1, 2). The ECD of mature, full-length FGFR3 shares 92% amino acid sequence identity with mouse FGFR3. Alternative splicing generates multiple forms of FGFR1-3, each with unique signaling characteristics (1-3). For FGFR3, alternative splicing of the ECD, specifically the IgIII domain, results in IIIb, or IIIc isoforms (4). The FGFR splice variants also exhibit distinct and varying binding affinities for different FGF ligands (2). FGFRs mediate the FGF signaling cascade which regulate developmental processes including cellular proliferation, differentiation, and migration, morphogenesis, and patterning (5). FGFRs transduce the signals through three dominant pathways including RAS/MAPK, PI3k/AKT, and PLC gamma (6). FGFR3 is normally expressed in the tissues of the central nervous system, brain, kidney and testis, with the FGFR3A(IIIb) splice variant predominantly expressed in epithelial cells (7, 8). FGFR3 signaling is critical for bone growth regulation and mutations in FGFR3 or misregulation of FGFR3 mediated signaling is found in multiple skeletal dysplasias, with FGFR3A(IIIb) specifically upregulated in hepatocellular carcinoma but down regulated in colorectal cancer (1, 4, 8, 9).

References

  1. Ornitz, D.M. and Itoh, N. (2015) Wiley Interdiscip Rev Dev Biol. 4:215.
  2. Zhang, X. et al. (2006) J Biol Chem. 281:15694.
  3. Ferguson, H.R. et al. (2021) Signaling. Cells 10:1201.
  4. Holzmann, K. et al. (2012) J Nucleic Acids. 2012:950508.
  5. Xie, Y. et al. (2020) Sig Transduct Target Ther 5:181.
  6. Mossahebi-Mohammadi, M. et al. (2020) Front Cell Dev Biol. 18:79.
  7. Sturla, L.M. et al. (2003) Br. J. Cancer 89:1276.
  8. Paur, J. et al. (2015) Hepatology. 62:1767.
  9. Teven, C.M. et al. (2014) Genes Dis. 1:199.

Long Name

Fibroblast Growth Factor Receptor 3

Alternate Names

CD333, CEK, FGF R3, JTK4

Entrez Gene IDs

2261 (Human); 14184 (Mouse)

Gene Symbol

FGFR3

UniProt

Additional FGFR3 Products

Product Documents for Recombinant Human FGFR3 alpha (IIIb) His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human FGFR3 alpha (IIIb) His-tag Protein, CF

For research use only

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