Recombinant Human FCRL1/FcRH1 Protein, CF

Catalog # 2049-FC | R&D Systems, Inc. a Bio-Techne Brand
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2049-FC-050
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Key Product Details

Accession #

Q96LA6

Source

NS0

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human FCRL1/FcRH1 protein
Ala17-Asn303, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala17

Predicted Molecular Mass

32 kDa

SDS-PAGE

38-45 kDa, reducing conditions

Activity

Measured by its ability to bind human IgG with an estimated KD

Formulation, Preparation and Storage

2049-FC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRL1/FcRH1

Fc Receptor-Like 1 (FCRL1), also known as FcRH1, and IRTA5, is an approximately 50 kDa protein with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRL1 binds to purified human IgG. Mature human FCRL1 consists of a 291 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 101 aa cytoplasmic domain with two immunotyrosine activation motifs (ITIMs) (3, 4). A charged glutamic acid within the transmembrane segment may mediate association with other signaling proteins. Alternative splicing may generate an isoform that lacks the transmembrane segment and an isoform that largely consists of the first two Ig-like domains (5).  Mouse FCRL1 contains only two Ig-like domains, but it shares 62% aa sequence identity with homologous regions of the human FCRL1 ECD. FCRL1 is expressed on pre-B cells and naive B cells (6, 7). It is downregulated upon B cell activation but upregulated on memory B cells (6, 8). FCRL1 is expressed on many B cell lymphoma and leukemia tumor cells with the exception of B cell acute lymphoblastic leukemia (9 - 11). Antibody crosslinking of FCRL1 triggers its tyrosine phosphorylation and augments B cell proliferation induced by the B cell receptor (6).

References

  1. Davis, R.S. et al. (2007) Annu. Rev. Immunol. 25:525.
  2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
  3. Miller, I. et al. (2002) Blood 99:2662.
  4. Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772.
  5. Accession # Q96LA6.
  6. Leu, C.-M. et al. (2005) Blood 105:1121.
  7. Li, F.J. et al. (2008) Blood 112:179.
  8. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
  9. Du, X. et al. (2008) Blood 111:338.
  10. Kazemi, T. et al. (2008) Int. J. Cancer 123:2113.
  11. Kazemi, T. et al. (2009) Cancer Immunol. Immunother. 58:989.

Long Name

Fc Receptor-like 1

Alternate Names

BXMH1, CD307a, FcRH1, IFGP1, IRTA5

Entrez Gene IDs

115350 (Human); 229499 (Mouse); 680665 (Rat)

Gene Symbol

FCRL1

UniProt

Q96LA6

Product Documents for Recombinant Human FCRL1/FcRH1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human FCRL1/FcRH1 Protein, CF

For research use only

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