Recombinant Human F13A1 His-tag Protein, CF

Name: Recombinant Human F13A1 His-tag Protein, CF
Brand: R&D Systems
SKU: 10179-F1

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 10179-F1

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Source:	Sf 21 (baculovirus)
Accession #:	NP_000120.2
Applications:	Enzyme Activity

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Product Specifications for Recombinant Human F13A1 His-tag Protein, CF

Source	Spodoptera frugiperda, Sf 21 (baculovirus)-derived human F13A1 protein Ser2-Met732 with an N-terminal Met and 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	No results obtained, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments.
Predicted Molecular Mass	84 kDa
SDS-PAGE	76-88 kDa, under reducing conditions
Activity	Measured by its ability to release DNP from Abz-NE(CAD-DNP)EQVSPLTLLK-OH. The specific activity is >13.0 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Human F13A1 His-tag Protein, CF

	Recombinant Human F13A1 His-tag Protein Enzyme Activity Recombinant Human F13A1 His-tag (Catalog # 10179-F1) is measured by its ability to release DNP from Abz-NE(CAD-DNP)EQVSPLTLLK-OH. The activity (orange) is approximately 2-fold higher than the competitor's F13A1 (green).
	Recombinant Human F13A1 His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Human F13A1 His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing a band at ~82 kDa under reducing conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10179-F1

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl, EDTA, Glycerol and TCEP.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

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Background: F13A1

Coagulation Factor XIIIa1 (F13a1) is a member of the transglutaminase family which includes F13A1 and TGM1-7 (1). F13 in the plasma is a tetrameric complex composed of two alpha (A) and two beta (B) chains where the A subunit is a transglutaminase zymogen and the B subunit is an inhibitory glycoprotein with no enzymatic function (2). Activation by thrombin and calcium ions results in the formation of the catalytically active transglutaminase F13a composed of an alpha chain homodimer capable of forming gamma-glutamyl-epsilon-lysine cross-links. The 83 kDa F13a monomer has an N-terminal activation peptide and a beta sandwich preceding the catalytic core with catalytic triad active site and two C-terminal beta barrels (3). The active homodimer is intracellular in platelets, megakaryocytes, monocytes and macrophages. The primary physiological outcome of the catalytic activity of F13a is cross-linking of fibrin and anti-plasmin to stabilize the fibrin clot (4,5). However, in addition to cross-linking fibrin, F13a is capable of cross-linking many substrates involved in complement activation, coagulation, inflammatory and immune responses and extracellular matrix organization (6). Cross-linking of key substrates by F13a has been directly shown to play a role in in atherosclerosis (7), wound healing (8), angiogenesis (9,10), maintaining pregnancy (11), ECM deposition, osteoblast differentiation and bone remodeling (12), and immune defense (13). F13A has also been detected as a marker in acute promyelocytic leukemia (APL)(14) and expression is considered of value for diagnosis and prognosis for leukemia-associated immunophenotype. Congenital deficiency results in bleeding manifestations including intercranial hemorrhage (15), poor wound healing (17), and spontaneous abortions (17) that can be treated with F13 (18).

Item	Value
References	Griffin, M. et al. (2002) Biochem. J. 368:377. Muszbek, L. et al. (1999) Thromb. Res. 94:271. Yee, V. C. et al. (1994) Proc. Natl. Acad. Sci. USA 91:7296. Lord, S. T. et al. (2011) Aterioscler. Thromb. Vasc. Biol. 31:494. Fraser, S. R. et al. (2011) Blood 117:6371. Nikolajsen, C. L. et al. (2014) J. Biol. Chem. 289:6526. AbdAlla, S. et al. (2004) Cell. 119:343. Nahrendorf, M. et al. (2006) Circulation 113:1196. Dardik, R. et al. (2006) Thromb. Haemost. 95:546. Dardik, R. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:526 Asahina, T. et al. (2000) Placenta. 21:388. Piercy-Kotb, S. A. et al. (2012) J. Cell Physiol. 227:2936. Richardson, V. R. et al. (2012) Br. J. Haematol. 160:116. Simon, A. et al. (2012) Cytometry B. Clin. Cytom. 82:209. Naderi, M. et al. (2015) Hematology. 20:112. Inbal, A. et al. (2005) Thromb. Haemost. 94:432. Inbal, A. and L. Muszbek. (2003) Semin. Thromb. Hemost. 29:171 Naderi, M. et al. (2016) Iran J. Pharm. Res. 15:635.
Long Name	Coagulation Factor XIII, A1 Polypeptide
Entrez Gene IDs	2162 (Human)
Alternate Names	Coagulation factor XIIIa, EC 2.3.2.13, F13A, F13A1, F13Acoagulation factor XIII, A polypeptide, FSF, A Subunit, Protein-glutamine gamma-glutamyltransferase A chain, TGase, Transglutaminase A chain, bA525O21.1 (coagulation factor XIII, A1 polypeptide), coagulation factor XIII A chain, coagulation factor XIII, A1 polypeptide, factor XIIIa, fibrin stabilizing factor, A subunit, fibrinoligase, transglutaminase. plasma

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Recombinant Human F13A1 His-tag Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human F13A1 His-tag Protein, CF

Scientific Data Examples for Recombinant Human F13A1 His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

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Background: F13A1

Citations for Recombinant Human F13A1 His-tag Protein, CF

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