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Recombinant Human Ephrin-A4 Fc Chimera Protein, CF
Catalog # 369-EA | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Accession #
Source
NS0
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Binding Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Ephrin-A4 protein
| Human Ephrin-A4 (Leu26 - Gly171) Accession # P52798 |
IEGRMD | Human IgG1 (Pro100 - Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Leu26
Predicted Molecular Mass
43.7 kDa (monomer)
SDS-PAGE
50 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
Immobilized rmEphA7/Fc Chimera at 2 µg/mL (100 µL/well) can bind rhEphrin-A4/Fc Chimera with a linear range of 0.16-10 ng/mL.
Immobilized rmEphA7/Fc Chimera at 2 µg/mL (100 µL/well) can bind rhEphrin-A4/Fc Chimera with a linear range of 0.16-10 ng/mL.
Formulation, Preparation and Storage
369-EA
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Ephrin-A4
Ephrin-A4, also known as LERK-4 and EFL-4, (1) is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A4 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1 (2, 3). The extracellular domains of human and mouse Ephrin-A4 share 80% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).
References
- Eph Nomenclature Committee [letter] (1997) Cell 90:403.
- Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
- Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.
Alternate Names
EFL-4, EFNA4, EphrinA4, LERK-4
Gene Symbol
EFNA4
UniProt
Product Documents for Recombinant Human Ephrin-A4 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human Ephrin-A4 Fc Chimera Protein, CF
For research use only
Citations for Recombinant Human Ephrin-A4 Fc Chimera Protein, CF (6)
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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Ephrin-A4 Fc Chimera Protein, CF include:
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Species: Human
Sample Types: Reference Standard
YL Chen et al. (2021-01-12), Ephrin A4-ephrin receptor A10 signaling promotes cell migration and spheroid formation by upregulating NANOG expression in oral squamous cell carcinoma cells Scientific Reports, 2021-011(1):644.
PMID: 33436772 -
Species: Virus - Henipavirus
Sample Types: Recombinant Protein
Applications: Coprecipitation AssayED Laing et al. (2019-09-23), Structural and functional analyses reveal promiscuous and species specific use of ephrin receptors by Cedar virus Proc. Natl. Acad. Sci. U.S.A., 2019-00(0).
PMID: 31548390 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayBaumer B et al. (2014-01-29), Nogo receptor homolog NgR2 expressed in sensory DRG neurons controls epidermal innervation by interaction with Versican. J Neurosci, 2014-034(5):1633-46.
PMID: 24478347 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayWilson TR et al. (2012-07-26), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-0487(7408):505-9.
PMID: 22763448 -
Species: Human
Sample Types: Whole Cells
Applications: ControlHahn AS et al. (2012-06-01), The ephrin receptor tyrosine kinase A2 is a cellular receptor for Kaposi's sarcoma-associated herpesvirus. Nat. Med., 2012-018(6):961-6.
PMID: 22635007 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayDawson DW et al. (2007-01-29), Global DNA methylation profiling reveals silencing of a secreted form of Epha7 in mouse and human germinal center B-cell lymphomas. Oncogene, 2007-026(29):4243-52.
PMID: 17260020
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