Recombinant Human EphB4 Protein, CF
Catalog # 3038-B4 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Product Specifications
Source
Leu16-Ala539, with a C-terminal 6-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
Immobilized rhEphB4 at 2 µg/mL (100 µL/well) can bind rmEphrin-B2/Fc Chimera with a linear range of 0.0780- 5.00 ng/mL.
Formulation, Preparation and Storage
3038-B4
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: EphB4
EphB4, also known as Htk, Myk1, Tyro11, and Mdk2, is a member of the Eph receptor tyrosine kinase family and binds Ephrin-B2. The A and B class Eph proteins have a common structural organization (1 - 4). The human EphB4 cDNA encodes a 987 amino acid precursor that includes a 15 amino acid (aa) signal sequence, a 524 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 427 aa cytoplasmic domain (5). The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) (5). Activation of kinase activity occurs after membrane-bound or clustered ligand recognition and binding. The ECD of human EphB4 shares 89% aa sequence identity with mouse EphB4 and 42 - 45% aa sequence identity with human EphB1, 2, and 3. EphB4 is expressed preferentially on venous endothelial cells (EC) and inhibits cell-cell adhesion, chemotaxis, and angiogenesis. Opposing effects are induced by signaling through Ephrin-B2 expressed on arterial EC: adhesion, endothelial cell migration, and vessel sprouting (6). EphB4 singaling contributes to new vascularization by guiding venous EC away from Ephrin-B2 expressing EC. Ephrin-B2 signaling induces arterial EC to migrate towards nascent EphB4 expressing vessels (6). The combination of forward signaling through EphB4 and reverse signaling through Ephrin-B2 promotes in vivo mammary tumor growth and tumor-associated angiogenesis (7). EphB4 promotes the differentiation of megakaryocytic and erythroid progenitors but not granulocytic or monocytic progenitors (8, 9).
References
- Poliakov, A. et al. (2004) Dev. Cell 7:465.
- Surawska, H. et al. (2004) Cytokine Growth Factor Rev. 15:419.
- Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
- Davy, A. and P. Soriano (2005) Dev. Dyn. 232:1.
- Bennett, B.D. et al. (1994) J. Biol. Chem. 269:14211.
- Fuller, T. et al. (2003) J. Cell Sci. 116:2461.
- Noren, N.K. et al. (2004) Proc. Natl. Acad. Sci. 101:5583.
- Wang, Z. et al. (2002) Blood 99:2740.
- Inada, T. et al. (1997) Blood 89:2757.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for Recombinant Human EphB4 Protein, CF
Product Specific Notices for Recombinant Human EphB4 Protein, CF
For research use only
Citations for Recombinant Human EphB4 Protein, CF (3)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human EphB4 Protein, CF include:
-
Species: Human
Sample Types: Antibody
Applications: Enzyme AssayZhang et al. (2015-04-13), EphB4 forward signalling regulates lymphatic valve development. Nat Commun, 2015-06(0):6625.
PMID: 25865237 -
Species: Mouse
Sample Types: Whole Cells
Applications: ICCWang Y et al. (2010-05-27), Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis. Nature, 2010-0465(7297):483-6.
PMID: 20445537 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayHe S et al. (2009-08-20), Soluble EphB4 inhibition of PDGF-induced RPE migration in vitro. Invest. Ophthalmol. Vis. Sci., 2009-051(1):543-52.
PMID: 19696168
There are no citations that match your criteria.
Customer Reviews for Recombinant Human EphB4 Protein, CF (1)
Reconstitution Calculator
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.