Recombinant Human DR6/TNFRSF21 Fc Chimera Protein, CF

Catalog # 144-DR | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #

O75509

Source

Sf 21 (baculovirus)

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human DR6/TNFRSF21 protein
Human DR6
(Gln42 - Leu350)
Accession # O75509		 DIEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln42

Predicted Molecular Mass

60.3 kDa (monomer)

SDS-PAGE

65-75 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When recombinant human APP770 is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Human DR6/TNFRSF21 that produces 50% of the optimal binding response is found to be approximately 20‑100 ng/mL.

Formulation, Preparation and Storage

144-DR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DR6/TNFRSF21

Death Receptor 6 (DR6), also known as TNFRSF21 and CD358, is a type I transmembrane protein in the TNF receptor superfamily (1). Human DR6 consists of a 308 amino acid (aa) extracellular domain (ECD) with four cysteine‑rich motifs, a 21 aa transmembrane segment, and a 285 aa palmitylated cytoplasmic region that contains one death domain (2, 3). Within the ECD, human and mouse DR6 share 82% aa sequence identity. DR6 is expressed as an approximately 110 kDa molecule that carries extensive N‑linked and O‑linked glycosylation in its extracellular region (3, 4). Among hematopoietic cells, DR6 is expressed on monocytes, resting CD4+ T cells, and pro‑, pre‑, and naïve B cells (5 ‑ 7). DR6 knockout mice exhibit a Th2‑biased immune response characterized by exaggerated Th2 and B cell responsiveness in combination with reduced Th1 cell responsiveness and inflammatory leukocyte infiltration (6 ‑ 9). DR6 knockout mice are resistant to induced airway inflammation and experimental autoimmune encephalitis but more susceptible to severe graft versus host disease (9 ‑ 11). DR6 is also expressed on developing neurons where it can bind a shed 35 kDa N‑terminal fragment of APP or a fragment of APLP2 (12, 13). This APP fragment is generated following deprivation of neurotrophic factors, and its binding to DR6 triggers DR6‑mediated axonal pruning (12). DR6 is constitutively expressed on some prostate cancer cells and can be induced by TNF‑ alpha on others (3, 4).

References

  1. Benschop, R. et al. (2009) Adv. Exp. Med. Biol. 647:186.
  2. Pan, G. et al. (1998) FEBS Lett. 431:351.
  3. Klima, M. et al. (2009) Biochim. Biophys. Acta 1793:1579.
  4. Kasof, G.M. et al. (2001) Oncogene 20:7965.
  5. Matesanz-Isabel, J. et al. (2011) Immunol. Lett. 134:104.
  6. Schmidt, C.S. et al. (2003) J. Exp. Med. 197:51.
  7. Liu, J. et al. (2001) Immunity 15:23.
  8. Zhao, H. et al. (2001) J. Exp. Med. 194:1441.
  9. Venkataraman, C. et al. (2006) Immunol. Lett. 106:42.
  10. Schmidt, C.S. et al. (2005) J. Immunol. 175:2286.
  11. Liu, J. et al. (2002) J. Immunol. 169:3993.
  12. Nikolaev, A. et al. (2009) Nature 457:981.
  13. Kuester, M. et al. (2011) J. Mol. Biol. 409:189.

Long Name

Death Receptor 6

Alternate Names

BM-018, CD358, TNFRSF21

Entrez Gene IDs

27242 (Human); 94185 (Mouse); 316256 (Rat)

Gene Symbol

TNFRSF21

UniProt

O75509

Product Documents for Recombinant Human DR6/TNFRSF21 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human DR6/TNFRSF21 Fc Chimera Protein, CF

For research use only

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