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Recombinant Human DLEC/CLEC4C/BDCA-2 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1376-DL

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1376-DL-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human DLEC/CLEC4C/BDCA-2 protein
Human IgG1
(Ser102-Lys330)
IEGR Human DLEC
(Phe46-Ile213)
Accession # Q8WTT0
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser102 (Fc)

Predicted Molecular Mass

46 kDa

SDS-PAGE

55-61 kDa, reducing conditions

Activity

Measured by its ability to bind fluorescein-conjugated S. aureus Bioparticles. Jiang, Y. et al. (2006) J. Biol. Chem. 281:11834.
The ED50 for this effect is 0.5-2.5 µg/mL.

Formulation, Preparation and Storage

1376-DL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLEC/CLEC4C/BDCA-2

Dendritic cell lectin (DLEC), also known as BDCA-2, CD303, HECL, and CLEC4C/CLECSF11/CLECSF7, is a 38 kDa type II transmembrane protein in the C-type lectin family (1). Mature human DLEC consists of a 21 amino acid (aa) cytoplasmic domain, a 23 aa transmembrane segment, and a 169 aa extracellular domain (ECD) that contains a juxtamembrane neck region and one carbohydrate recognition domain (CRD) (2, 3). Alternate splicing may generate multiple isoforms that lack the transmembrane segment and/or portions of the cytoplasmic, neck, and CRD regions (2 - 4). An ortholog of human DLEC has not been described in mouse or rat. DLEC expression is restricted to plasmacytoid dendritic cells (pDC) and is downregulated during their maturation (2, 3, 5). pDC play a role in the innate immune response by producing IFN-alpha / beta following exposure to TLR7 and TLR9 agonists such as microbial CpG DNA (3, 5 - 8). Antibody ligation of DLEC on pDC attenuates the CpG-stimulated production of interferons as well as a Th1 biased response (3, 5 - 9). DLEC interactions with HIV-1 gp120 and hepatitis B virus soluble antigen may therefore limit the pDC antiviral response (10, 11). Similar to other C-type lectins, DLEC can mediate antigen uptake for MHC loading and presentation to T cells (3, 12). Crosslinking of DLEC on CpG-stimulated pDC inhibits pDC maturation and induces tyrosine phosphorylation on multiple proteins involved in B cell receptor signaling and endocytosis (5, 7, 8). These functions require the association of DLEC with the ITAM-containing Fc epsilon RI gamma chain (7, 8).

References

  1. Kanazawa, N. et al. (2004) Immunobiology 209:179.
  2. Arce, I. et al. (2001) Eur. J. Immunol. 31:2733.
  3. Dzionek, A. et al. (2001) J. Exp. Med. 194:1823.
  4. Fernandes, M.J. et al. (2000) Genomics 69:263.
  5. Wu, P. et al. (2008) Clin. Immunol. 129:40.
  6. Fanning, S.L. et al. (2006) J. Immunol. 177:5829.
  7. Rock, J. et al. (2007) Eur. J. Immunol. 37:3564.
  8. Cao, W. et al. (2007) PloS Biol. 5:e248.
  9. Riboldi, E. et al. (2009) Immunobiology 214:868.
  10. Martinelli, E. et al. (2007) Proc. Natl. Acad. Sci. 104:3396.
  11. Xu, Y. et al. (2009) Mol. Immunol. 46:2640.
  12. Jaehn, P.S. et al. (2008) Eur. J. Immunol. 38:1822.

Long Name

Dendritic Cell Lectin

Alternate Names

BDCA-2, BDCA2, CD303, CLEC4C, CLECSF11, CLECSF7

Entrez Gene IDs

170482 (Human)

Gene Symbol

CLEC4C

UniProt

Additional DLEC/CLEC4C/BDCA-2 Products

Product Documents for Recombinant Human DLEC/CLEC4C/BDCA-2 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human DLEC/CLEC4C/BDCA-2 Fc Chimera Protein, CF

For research use only

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