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Recombinant Human Desert Hedgehog/Dhh (C23II) N-Terminus, CF

Bio-Techne includes R&D Systems | Catalog # 4777-DH

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Key Product Details


E. coli

Accession #





Product Specifications


E. coli-derived human Desert Hedgehog/Dhh protein
Cys23-Gly198 (Cys23Ile-Ile), with and without an N-terminal Met


>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met & Ile-Ile

Predicted Molecular Mass

20 kDa


Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465.
The ED50 for this effect is 15-45 µg/mL.

Formulation, Preparation and Storage

Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and DTT with Trehalose.
Reconstitute at 500 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desert Hedgehog/Dhh

Desert Hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Desert Hedgehog is a secreted, 45 kDa, 373 amino acid (aa) protein that undergoes autocatalytic cleavage between Gly198 and Cys199 catalyzed by the C-terminal domain, which releases the N-terminal domain with a concominant attachment of cholesterol at its new C-terminus. In addition to the C-terminally attached cholesterol, a fatty acid acyl chain is esterified to the N-terminal cysteine (aa 23) via an amide linkage. The 19 kDa N-terminal signaling domain is membrane associated due to its double lipid modifications. Its binding to Patched receptors results in the loss of Patched repression of Smoothened signaling (1 - 4). Dhh binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip) (5). Within the N-terminal domain, human Dhh shares 97% aa sequence identity with mouse and rat Dhh. It shares approximately 75% aa seqeuence identity with human Indian (Ihh) and Sonic Hedgehog (Shh) (5). Dhh is produced by Sertoli cells and is required for testis development and spermatogenesis (6, 7). It induces steroidogenic factor 1, which is instrumental in promoting Leydig cell differentiation (8, 9). It also promotes the deposition of basal lamina surrounding seminiferous tubules (6). Mutations of Dhh are linked to complete pure gonadal dysgenesis (10). Dhh is expressed in the female by ovarian granulosa cells and the corporus luteum (11). Its upregulation in ovarian cancer correlates positively with proliferative index, and negatively with prognosis (12). Dhh is also expressed by Schwann cells and is upregulated following nerve injury (13, 14). It induces the expression of Patched and Hip in neural tissue fibroblasts and promotes the formation of the connective tissue sheath surrounding peripheral nerves (13).


  1. van den Brink, G.R. (2007) Physiol. Rev. 87:1343. 
  2. Riobo, N.A. and D.R. Manning (2007) Biochem. J. 403:369. 
  3. Porter, J.A. et al. (1995) Nature 374:363. 
  4. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. 95:13630. 
  5. Pathi, S. et al. (2001) Mech. Dev. 106:107. 
  6. Pierucci-Alves, F. et al. (2001) Biol. Reprod. 65:1392. 
  7. Bitgood, M.J. et al. (1996) Curr. Biol. 6:298. 
  8. Yao, H.H-C. et al. (2002) Genes Dev. 16:1433.
  9. Park, S.Y. et al. (2007) Endocrinology 148:3704.
  10. Canto, P. et al. (2004) J. Clin. Endocrinol. Metab. 89:4480.
  11. Russell, M.C. et al. (2007) Biol. Reprod. 77:226.
  12. Chen, X. et al. (2007) Cancer Sci. 98:68.
  13. Parmantier, E. et al. (1999) Neuron 23:713.
  14. Bajestan, S.N. et al. (2006) J. Neurobiol. 66:243.

Alternate Names


Entrez Gene IDs

50846 (Human); 13363 (Mouse)

Gene Symbol



Additional Desert Hedgehog/Dhh Products

Product Documents for Recombinant Human Desert Hedgehog/Dhh (C23II) N-Terminus, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Desert Hedgehog/Dhh (C23II) N-Terminus, CF

For research use only

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