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Recombinant Human CXCL9/MIG Protein, CF

Catalog # 11106-MG | R&D Systems, Inc. a Bio-Techne Brand
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11106-MG-050

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CXCL9/MIG protein
Thr23-Thr125

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr23

Predicted Molecular Mass

12 kDa

SDS-PAGE

12-17 kDa, under reducing conditions.

Activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CXCR3. The ED50 for this effect is 0.020‑0.300 µg/mL.

Scientific Data Images for Recombinant Human CXCL9/MIG Protein, CF

Recombinant Human CXCL9/MIG Protein Bioactivity.

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CXCR3. The ED50 for this effect is 0.020‑0.300 µg/mL.

Recombinant Human CXCL9/MIG Protein SDS-PAGE.

2 μg/lane of Recombinant Human CXCL9/MIG Protein (Catalog # 11106-MG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 12-17 kDa.

Formulation, Preparation and Storage

11106-MG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL9/MIG

CXCL9, a member of the alpha subfamily of chemokines that lack the ELR domain, was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse MIG cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T‑lymphocytes and TIL but not for neutrophils or monocytes. The human CXCL9 cDNA encodes a 125 amino acid residue precursor protein with a 22 amino acid residue signal peptide that is cleaved to yield a 103 amino acid residue mature protein. CXCL9 has an extended carboxy‑terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. The carboxy‑terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy‑terminal deletions have been shown to have diminished activity in the calcium flux assay. MIG also functions in vivo as a potent chemoattractant for tumor-infiltrating lymphocytes and activates peripheral blood lymphocytes, as well as NK cells and TH1 lymphocytes. Therefore, MIG could be a potential candidate for antiangiogenic and immunomodulation therapy for tumor disease. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T‑lymphocytes.

References

  1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
  2. Liao, F. et al. (1995) J. Exp. Med. 182:1301.
  3. Vanguri, P. (1995) J. Neuroimmunol. 56:35.
  4. Zhang, R. et al. (2006) Gene Ther. 13:1263.

Alternate Names

MIG

Entrez Gene IDs

4283 (Human); 17329 (Mouse); 246759 (Rat)

Gene Symbol

CXCL9

UniProt

Product Documents for Recombinant Human CXCL9/MIG Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CXCL9/MIG Protein, CF

For research use only

Reconstitution Calculator

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