Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF Best Seller

Catalog # E3-650 | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
Availability
Size / Price
Qty
E3-650-025
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Accession #

Q13619.3 (CUL4A); P62877.1 (RBX1); Q16531.1 (DDB1), Q96SW2.1 (CRBN)

Source

Sf 21 (baculovirus)

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL4A/RBX1/DDB1/CRBN Complex protein
This complex consists of an N-terminal 10-His tagged Cullin-4A, untagged RBX1, untagged DDB1 and untagged CRBN.

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

94 kDa (CUL4A), 12 kDa (RBX1), 127 kDa (DDB1), 51 kDa (CRBN)

Activity

Typical concentration to support in vitro conjugation reactions will depend on experimental conditions.

Formulation, Preparation and Storage

E3-650
Formulation Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: CUL4A/RBX1/DDB1/CRBN Complex

Cullin-4A (CUL4A) is a core component of multiple cullin-RING type Ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cell cycle progression, DNA repair and other processes.  In the DCX complex (DDB1-CUL4-X-box), CUL4A serves as a scaffold that organizes the DDB1-X-box substrate recognition subunits with the RBX1 subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  In vivo, the E3 ubiquitin ligase activity of the DCX complex is dependent on neddylation of the CUL4 subunit, though neddylation may be dispensable in some in vitro reactions.  

References

  1. Fischer E.S., et al.  (2014) Nature  512: 49.
  2. He Y.J., et al.  (2006) Genes Dev.  20: 2949.
  3. Ito T., et al.  (2010) Science  327: 1345.
  4. Wang H., et al.  (2006) Mol. Cell  22: 383.

Long Name

Cullin-4A/RING Box Protein 1/Damage Specific DNA Binding Protein 1/Cereblon Complex

UniProt

Q13619.3 (CUL4A); P62877.1 (RBX1); Q16531.1 (DDB1), Q96SW2.1 (CRBN)

Product Documents for Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF

For research use only

FAQs for Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Can I purchase CRBN separately?

    A: At this time, CRBN is only available as a complex. If you are interested in a custom, stand-alone CRBN protein, please contact Custom Services (https://www.rndsystems.com/services).

  • Q: What is the main difference between the Recombinant Human CUL4A/NEDD8/RBX1/DDB1/CRBN Protein, CF (Catalog # E3-651) and the Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF (Catalog # E3-650)?

    A: The main difference between these products is that Catalog # E3-651 includes NEDD8 in the complex with CUL4A, RBX1, DDB1, and CRBN. CUL4A is neddylated, or attached to NEDD8. Catalog # E3-650 contains all of the same proteins in a complex (except NEDD8), but CUL4A is not neddylated.

    For this complex, neddylation is required for ligase activity in vivo, but generally not for in vitro activity. For in vitro applications, both complexes should work similarly and Catalog # E3-651 should be able to perform all of the functions of Catalog # E3-650.

Showing  1 - 2 of 2 FAQs Showing All
Setting the standard in quality research reagents