Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF Best Seller
Catalog # E3-650 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Source
Conjugate
Applications
Product Specifications
Source
This complex consists of an N-terminal 10-His tagged Cullin-4A, untagged RBX1, untagged DDB1 and untagged CRBN.
Purity
Predicted Molecular Mass
Activity
Formulation, Preparation and Storage
E3-650
Formulation | Supplied as a solution in HEPES, NaCl, DTT and Glycerol. |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: CUL4A/RBX1/DDB1/CRBN Complex
References
- Fischer E.S., et al. (2014) Nature 512: 49.
- He Y.J., et al. (2006) Genes Dev. 20: 2949.
- Ito T., et al. (2010) Science 327: 1345.
- Wang H., et al. (2006) Mol. Cell 22: 383.
Long Name
Product Documents for Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF
Product Specific Notices for Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF
For research use only
FAQs for Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF
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Q: Can I purchase CRBN separately?
A: At this time, CRBN is only available as a complex. If you are interested in a custom, stand-alone CRBN protein, please contact Custom Services (https://www.rndsystems.com/services).
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Q: What is the main difference between the Recombinant Human CUL4A/NEDD8/RBX1/DDB1/CRBN Protein, CF (Catalog # E3-651) and the Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF (Catalog # E3-650)?
A: The main difference between these products is that Catalog # E3-651 includes NEDD8 in the complex with CUL4A, RBX1, DDB1, and CRBN. CUL4A is neddylated, or attached to NEDD8. Catalog # E3-650 contains all of the same proteins in a complex (except NEDD8), but CUL4A is not neddylated.
For this complex, neddylation is required for ligase activity in vivo, but generally not for in vitro activity. For in vitro applications, both complexes should work similarly and Catalog # E3-651 should be able to perform all of the functions of Catalog # E3-650.