Recombinant Human CD38 Fc Chimera Protein, CF

Name: Recombinant Human CD38 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10920-AC

Catalog # 10920-AC

Product Datasheet / COA / SDS

Order Information

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Key Product Details

Source:	HEK293
Accession #:	P28907.2
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Human CD38 Fc Chimera Protein, CF

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD38 protein

Human CD38 (Val43-Ile300) Accession # P28907.2	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Val43

Predicted Molecular Mass

57 kDa

SDS-PAGE

67-74 kDa, under reducing conditions

Activity

Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD⁺) to cyclic GDP-ribose. Graeff, R.M. et al. (1994) J. Biol. Chem. 269:30260. The specific activity is >900 pmol/min/μg, as measured under the described conditions.

Recombinant Human CD38 Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Human CD38 Fc Chimera Protein SDS-PAGE. 2 μg/lane of Recombinant Human CD38 Fc Chimera Protein (Catalog # 10920-AC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 67-74 kDa and 130-150 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10920-AC

Formulation	Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD38

CD38, also known as ADP-ribosyl cyclase, converts NAD(P)+ into three separate products with calcium mobilizing ability: cyclic ADP-ribose, NAADP+, and ADP-ribose (1). CD38 is a Type II transmembrane glycoprotein composed of an intracellular domain, a single transmembrane helix domain, and a large extracellular domain that contains the catalytic site (2). CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells (3,4). Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells (5,6). CD38 is also reported to bind as a receptor to trigger signaling cascades (7,8). Through both mechanisms, CD38 influences proliferation and trafficking (8,9). CD38 is used as a marker for poor prognosis in chronic lymphocytic leukemia and multiple myeloma and is an attractive cancer immunotherapy drug target (8-11).

Item	Value
References	Schuber, F. and F.E. Lund (2004) Curr. Mol. Med. 4:249. Liu, Q. et al. (2005) Structure. 13:1331. Jackson, D.G. and J.I. Bell (1990) J. Immunol. 144:2811. Sun, L. et al. (1999) J. Cell Biol. 146:1161. Partida-Sanchez, S. et al. (2001) Nature Med. 7:1209. Kato, I. et al. (1995) J. Biol. Chem. 270:30045. Deaglio, S. (2000) Chem. Immunol. 75:99. Chillemi,A. et al. (2013) Mol. Med. 19:99. Vaisitti, T. et al. (2015) Leukemia. 29:356. Atanackovic, D. et al. (2016) Oncoimmunology. 5:e1217374. Tai, Y-T. et al. (2017) Oncotarget. 8:112166.
Long Name	Cluster of Differentiation 38
Entrez Gene IDs	952 (Human); 12494 (Mouse); 25668 (Rat); 102126394 (Cynomolgus Monkey)
Alternate Names	2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase (EC:2.4.99.20), 2'-phospho-cyclic-ADP-ribose transferase, ADP-ribosyl Cyclase, ADP-ribosyl cyclase 1, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, ADPRC 1, ADPRC1, CD38, CD38 antigen, CD38 antigen (p45), CD38 molecule, CD_antigen: CD38, Cyclic ADP-ribose Hydrolase, Cyclic ADP-ribose hydrolase 1, EC 2.4.99.20, EC 3.2.2.6, EC:3.2.2.6, NAD(+) nucleosidase, T10, cADPr hydrolase 1, cluster of differentiation 38

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