Recombinant Human CD177 Protein, CF

Catalog # 3505-CD | R&D Systems, Inc. a Bio-Techne Brand
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3505-CD-050
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Key Product Details

Accession #

AAH29167

Source

NS0

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CD177 protein
Leu22-Gly407, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu22

Predicted Molecular Mass

42 kDa

SDS-PAGE

57-63 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of EOMA mouse endothelial cells.
The ED50 for this effect is 0.3-1.5 μg/mL

Formulation, Preparation and Storage

3505-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD177

CD177, also known as NB1, human neutrophil antigen-2a (HNA-2a), and polycythemia rubra vera-1 (PRV-1), is an approximately 50-60 kDa glycoprotein member of the uPAR/CD59/Ly6 superfamily (1). Mature human CD177 is a 387 amino acid (aa) protein that contains two uPAR/Ly6 domains (2, 3). It lacks the first two uPAR domains that are present in mouse and rat CD177. Mature human CD177 shares 55% aa sequence identity with common regions of mouse and rat CD177. Alternative splicing of human CD177 generates additional isoforms that are truncated either just before or just after the first uPAR domain. CD177 is expressed on the surface of neutrophils through a glycosylphosphatidylinositol (GPI) anchor (2, 4, 5). It is nearly absent from neutrophils from paroxysmal nocturnal hemoglobinurea patients who are deficient in the ability to synthesize GPI linkages (5, 7). It is up-regulated on granulocytes from polycythemia vera and thalassemia patients (3, 6). CD177 binds to PECAM-1 on vascular endothelial cells, an interaction which mediates neutrophil adhesion to the vascular wall and neutrophil transmigration (8). It associates in cis with the Integrin MAC-1 (CD11b/CD18) (9). CD177 also associates in cis with Proteinase 3 (PR3) and is required for cell surface PR3 expression (9-11). PR3 is normally found in intracellular vesicles, but once at the cell surface it can serve as an autoimmune target for anti-neutrophil cytoplasmic antibodies (ANCA) (12). The ANCA targeting of CD177-PR3 complexes triggers neutrophil activation and vasculitis (9, 12).

References

  1. Stroncek, D.F. (2007) Curr. Opin. Hematol. 14:688.
  2. Kissel, K. et al. (2001) Eur. J. Immunol. 31:1301.
  3. Temerinac, S. et al. (2000) Blood 95:2569.
  4. Skubitz, K.M. et al. (1991) J. Leukoc. Biol. 49:163.
  5. Klippel, S. et al. (2002) Blood 100:2441.
  6. Zoi, K. et al. (2008) Brit. J. Haematol. 141:100.
  7. Boccuni, P. et al. (2000) Crit. Rev. Oncol. Hematol. 33:25.
  8. Sachs, U.J.H. et al. (2007) J. Biol. Chem. 282:23603.
  9. Jerke, U. et al. (2011) J. Biol. Chem. 286:7070.
  10. von Vietinghoff, S. et al. (2007) Blood 109:4487.
  11. Kuckleburg, C.J. et al. (2012) J. Immunol. 188:2419.
  12. van Timmeren, M.M. and P. Heeringa (2012) Curr. Opin. Rheumatol. 24:8.

Long Name

Polycythemia Rubra Vera Protein 1

Alternate Names

CD177, HNA-2a, NB1 GP, PRV1

Entrez Gene IDs

57126 (Human); 68891 (Mouse)

Gene Symbol

CD177

UniProt

AAH29167

Product Documents for Recombinant Human CD177 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human CD177 Protein, CF

For research use only

Reconstitution Calculator

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