Recombinant Human Cathepsin E Protein, CF

Name: Recombinant Human Cathepsin E Protein, CF
Brand: R&D Systems
SKU: 1294-AS
Price: 394 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1294-AS

Citations (3)

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Source:	NS0
Accession #:	NP_001901
Structure / Form:	Pro and mature forms
Applications:	Enzyme Activity

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Product Specifications for Recombinant Human Cathepsin E Protein, CF

Source	Mouse myeloma cell line, NS0-derived human Cathepsin E protein Gln18-Pro396, with a C-terminal 10-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ile54 & Gln18 (predicted)
Predicted Molecular Mass	42 kDa
SDS-PAGE	48 kDa, reducing conditions
Activity	Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH₂ (Catalog # ES001). The specific activity is >1,500 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1294-AS

Formulation	Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile 25 mM MES, 150 mM NaCl, pH 6.5.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cathepsin E

Cathepsin E is an intracellular aspartic protease of the pepsin family (1). Unlike Cathepsin D, another member of the same family and a lysosomal protease with relatively ubiquitous distribution, Cathepsin E is not a lysosomal enzyme and has a limited cell and tissue distribution. However, both Cathepsin D and E play an important role in the degradation of proteins, the generation of bioactive proteins, and antigen processing (2). Both enzymes are efficient in cleaving Swedish mutant of amyloid precursor protein (APP) at the beta site but show almost no reactivity with wild-type APP (3). Human Cathepsin E is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑17), a propeptide (residues 18‑53), and a mature chain (residues 54‑396) (4).

Item	Value
References	Kay, J. and P.J. Tatnell (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al. eds.), p. 33, Academic Press, San Diego. Tsukuba, T. et al. (2000) Mol. Cells 10:601. Gruninger-Leitch, F. et al. (2000) Nat. Biotechnol. 18:66. Azuma, T. et al. (1989) J. Biol. Chem. 264:16748.
Entrez Gene IDs	1510 (Human); 13034 (Mouse)
Alternate Names	CATE, CTSE, Cathepsin E, EC 3.4.23, EC 3.4.23.34, erythrocyte membrane aspartic proteinase, slow-moving proteinase

Citations for Recombinant Human Cathepsin E Protein, CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Cathepsin E Protein, CF include:

Species: Human
Sample Types: Protein
Applications: Bioassay

A Gilg et al. (2021), Absence of the CXCR4 antagonist EPI-X4 from pharmaceutical human serum albumin preparations Journal of Translational Medicine, 19(1):190.
PMID: 33941197
Applications: Bioassay

SL Ivry et al. (2017), Global protease activity profiling provides differential diagnosis of pancreatic cysts Clin. Cancer Res., 0(0).
PMID: 28424202
Species: Human
Sample Types: Tissue Homogenates
Applications: Bioassay

Zhou W et al. (2006), Cathepsin D-mediated proteolysis of apolipoprotein E: possible role in Alzheimer's disease. Neuroscience, 143(3):689-701.
PMID: 16997486

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Recombinant Human Cathepsin E Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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