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Recombinant Human Cathepsin E Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1294-AS

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1294-AS-010

Key Product Details

Source

NS0

Accession #

Structure / Form

Pro and mature forms

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Cathepsin E protein
Gln18-Pro396, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile54 & Gln18 (predicted)

Predicted Molecular Mass

42 kDa

SDS-PAGE

48 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001).
The specific activity is >1,500 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

1294-AS
Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile 25 mM MES, 150 mM NaCl, pH 6.5.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin E

Cathepsin E is an intracellular aspartic protease of the pepsin family (1). Unlike Cathepsin D, another member of the same family and a lysosomal protease with relatively ubiquitous distribution, Cathepsin E is not a lysosomal enzyme and has a limited cell and tissue distribution. However, both Cathepsin D and E play an important role in the degradation of proteins, the generation of bioactive proteins, and antigen processing (2). Both enzymes are efficient in cleaving Swedish mutant of amyloid precursor protein (APP) at the beta site but show almost no reactivity with wild-type APP (3). Human Cathepsin E is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑17), a propeptide (residues 18‑53), and a mature chain (residues 54‑396) (4).

References

  1. Kay, J. and P.J. Tatnell (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al. eds.), p. 33, Academic Press, San Diego.
  2. Tsukuba, T. et al. (2000) Mol. Cells 10:601.
  3. Gruninger-Leitch, F. et al. (2000) Nat. Biotechnol. 18:66.
  4. Azuma, T. et al. (1989) J. Biol. Chem. 264:16748.

Alternate Names

CTSE

Entrez Gene IDs

1510 (Human); 13034 (Mouse)

Gene Symbol

CTSE

UniProt

Additional Cathepsin E Products

Product Documents for Recombinant Human Cathepsin E Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Cathepsin E Protein, CF

For research use only

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