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Recombinant Human Carbonic Anhydrase VA Protein, CF

Catalog # 3049-CA | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #


E. coli




Enzyme Activity

Product Specifications


E. coli-derived human Carbonic Anhydrase VA/CA5A protein
Ala40-Ser305, with a C-terminal 10-His tag


>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis


Predicted Molecular Mass

31 kDa


32 kDa, reducing conditions


Measured by its esterase activity.
The specific activity is >100 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Carbonic Anhydrase VA/CA5A

Carbonic Anhydrase catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20 - 25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Carbonic Anhydrase VA encoded by the CA5A gene is a mitochondrial protein (2, 3). In comparison with another mitochondrial CA (CA5B), CA5A has different tissue distribution and chromosomal location (4, 5). Expression and inhibitor studies of different CAs in the rat pancreatic beta cells indicate that CA5A may be involved in the regulation of insulin secretion (6). CA5A may also participate in the detoxification of ammonia produced in the gastrointestinal tract by providing bicarbonate to carbamyl phosphate synthetase I (7). The amino acid sequence of recombinant human CA5A (residues 40 to 305) is 79%, 77%, and 76% identical to that of canine, bovine, and rat/mouse.


  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Nagao, Y. et al. (1993) Proc. Natl. Acad. Sci. USA 90:7623.
  3. Nagao, Y. et al. (1995) Genomics 28:477.
  4. Shah, G.N. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1677.
  5. Fujikawa-Adachi, K. et al. (1999) J. Biol. Chem. 274:21228.
  6. Parkkila, A.K. et al. (1998) J. Biol. Chem. 273:24620.
  7. Saarnio, J. et al. (1999) J. Histochem. Cytochem. 47:517.

Alternate Names


Entrez Gene IDs

763 (Human); 54233 (Rat)

Gene Symbol



Product Documents for Recombinant Human Carbonic Anhydrase VA Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Carbonic Anhydrase VA Protein, CF

For research use only

Setting the standard in quality research reagents