Key Product Details
Ala40-Ser305, with a C-terminal 10-His tag
N-terminal Sequence Analysis
Predicted Molecular Mass
The specific activity is >100 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
|Formulation||Supplied as a 0.2 μm filtered solution in Tris and NaCl.|
|Shipping||The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Carbonic Anhydrase VA/CA5A
Carbonic Anhydrase catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20 - 25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Carbonic Anhydrase VA encoded by the CA5A gene is a mitochondrial protein (2, 3). In comparison with another mitochondrial CA (CA5B), CA5A has different tissue distribution and chromosomal location (4, 5). Expression and inhibitor studies of different CAs in the rat pancreatic beta cells indicate that CA5A may be involved in the regulation of insulin secretion (6). CA5A may also participate in the detoxification of ammonia produced in the gastrointestinal tract by providing bicarbonate to carbamyl phosphate synthetase I (7). The amino acid sequence of recombinant human CA5A (residues 40 to 305) is 79%, 77%, and 76% identical to that of canine, bovine, and rat/mouse.
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Nagao, Y. et al. (1993) Proc. Natl. Acad. Sci. USA 90:7623.
- Nagao, Y. et al. (1995) Genomics 28:477.
- Shah, G.N. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1677.
- Fujikawa-Adachi, K. et al. (1999) J. Biol. Chem. 274:21228.
- Parkkila, A.K. et al. (1998) J. Biol. Chem. 273:24620.
- Saarnio, J. et al. (1999) J. Histochem. Cytochem. 47:517.
Product Specific Notices for Recombinant Human Carbonic Anhydrase VA Protein, CF
For research use only