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Recombinant Human Carbonic Anhydrase IV Protein, CF

Bio-Techne includes R&D Systems | Catalog # 2186-CA

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2186-CA-010

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Carbonic Anhydrase IV/CA4 protein
Ala19-Lys283, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala19

Predicted Molecular Mass

32 kDa

SDS-PAGE

33 kDa, reducing conditions

Activity

Measured by its esterase activity.
The specific activity is >2 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

2186-CA
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Carbonic Anhydrase IV/CA4

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20 - 25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA4 is a GPI‑anchored membrane enzyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and of proximal renal tubules. It functions as the principal CO2 taste sensor (2). In addition, a genetic mutation (Arg 14 to Trp in the signal peptide) of CA4 was found to cosegregate with the RP17 form of retinitis pigmentosa in two large families and was not found in 36 unaffected family members or 100 controls (3).

References

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Chandrashekar, J. et al. (2009) Science 326:443.
  3. Rebello, G. et al. (2004) Proc. Natl. Acad. Sci. USA 101:6617.

Alternate Names

CA4, RP17

Entrez Gene IDs

762 (Human); 12351 (Mouse)

Gene Symbol

CA4

UniProt

Additional Carbonic Anhydrase IV/CA4 Products

Product Documents for Recombinant Human Carbonic Anhydrase IV Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Carbonic Anhydrase IV Protein, CF

For research use only