Recombinant Human B-Raf kinase domain His-tag Protein, CF New

Name: Recombinant Human B-Raf kinase domain His-tag Protein, CF
Brand: R&D Systems
SKU: 11831-BR

R&D Systems, part of Bio-Techne | Catalog # 11831-BR

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Product Datasheet / COA / SDS

Key Product Details

Source:	Sf 21 (baculovirus)
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Human B-Raf kinase domain His-tag Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human B-Raf kinase domain His-tag Protein, CF

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human B-Raf protein

Met	6-His tag	Sumo-tag (mutated, uncleavable)	3c Protease site	Human B-Raf (Leu416-His766) Accession # P15056.4
N-terminus				C-terminus

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Protein identity confirmed by mass spectrometry

Predicted Molecular Mass

53 kDa

SDS-PAGE

53 kDa

Activity

Measured by its ability to transfer phosphate from adenosine triphosphate (ATP) to a synthetic peptide substrate.

The specific activity is >20 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Human B-Raf kinase domain His-tag Protein, CF

	Recombinant Human B‑Raf kinase domain His-tag Enzyme Activity. Recombinant Human B‑Raf kinase domain His-tag (Catalog # 11831-BR) is measured by its ability to transfer phosphate from adenosine triphosphate (ATP) to a synthetic peptide substrate.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11831-BR

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Mass (in vial)

Desired Reconstitution Concentration

Background: B-Raf

Serine/Threonine-protein kinase B-Raf (B-Raf), also referred to as proto-oncogene B-Raf and v-Raf murine sarcoma viral oncogene homolog B is a broadly expressed cytoplasmic kinase from the TKL (Tyrosine Kinase-Like) group of Serine/Threonine protein kinase family that functions as a critical gatekeeper in the MAP kinase/ERKs signaling pathway that controls cell division, proliferation and survival (1,2). B-Raf is one of three highly conserved RAF kinase isoforms that are composed of three conserved domains characteristic of the subfamily (3). Conserved region 1 (CR1) contains a Ras-GTP-binding self-regulatory domain that autoinhibits B-Raf's kinase domain thus regulating B-Raf signaling (3,4). Conserved region 2 (CR2) is a serine-rich hinge region, and conserved region 3 (CR3), is a conserved bi-lobal catalytic protein kinase domain where the smaller lobe contains the P-loop responsible for ATP binding and the larger lobe contains the activation loop that binds substrate proteins with the active site located in the cleft between the two lobes (5). B-Raf must bind Ras-GTP which causes a conformational change to its active dimeric conformation and phosphorylation (6,7). Although similar in structure, B-Raf is the Raf family member that has been shown to function as the main and most potent activator of the MAPK cascade via targeted MEK phosphorylation (3,8). Inherited mutations in the BRAF gene can cause Cardiofaciocutaneous, Noonan, and Leopard syndromes (9). More than 30 mutations of the BRAF gene, including the V600E mutation within the activation segment that causes constitutive signaling, have been associated with diverse human cancer types including colorectal cancer, melanoma, papillary thyroid carcinoma, non-small-cell lung carcinoma, glioblastoma (10-14), as well as inflammatory diseases such as Erdheim–Chester disease (1,2,15). As BRAF is a well-understood target (16) and inhibition via approved drugs targeting B-Raf specifically and in combination have shown improved survival and response rate for several mutation-related cancers including solid tumors (1,17,18,19), there is significant ongoing research in development of additional pharmacological inhibitors for B-Raf kinase.

Item	Value
References	Poulikakos, P. et al. (2022) Clin. Cancer Res. 28:4618. Mechahougui, H. et al. (2025) Int. J. Sci. 26:3757. Daum, G. et al. (1994) Trends Biochem. Sci. 19:474. Cutler Jr, R.E. et al. (1998) Proc. Natl. Acad. Sci. USA 95:9214. Hanks, S.K. and Hunter, T. (1995) FASEB J. 9:576. Morrison, D.K. and Cutler, R.E. (1997) Curr. Opin. Cell Biol. 9:174. Lavoie, H. and Therrien, M. (2015) Nat. Rev. Mol. Cell Biol. 16:281. Baccarini, M. (2005) FEBS Lett. 579:3271. Sarkozy, A. et al. (2009) Hum. Mutat. 30:695. Davies, H. et al. (2002) Nature 417:949. Marchetti, A. et al. (2011) J. Clin. Oncol. 29:3574. Choi, S. et al. (2016) Int. J. Radiat. Oncol. Biol. Phys. 96:E356. Chu, J.E. et al. (2020) Clin. Cancer Res. 26:4599. Lim-Fat, M.J. et al. (2021) J. Neurooncol. 152:515. Haroche, J. et al. (2017) Lancet Oncol. 18:e113. King, A.J. et al. (2006) Cancer Res. 66:11100. Chapman, P.B. et al. (2011) N. Engl. J. Med. 364:2507. Barbato, M.I. et al. (2024) Clin. Cancer Res. 30:263. Hanrahan, A.J. et al. (2024) Nat. Rev. Clin. Oncol. 21:224.
Long Name	v-Raf Murine Sarcoma Viral Oncogene Homolog B1
Entrez Gene IDs	673 (Human); 114486 (Rat)
Alternate Names	94 kDa B-raf protein, B-RAF1, B-Raf, B-Raf proto-oncogene serine/threonine-protein kinase (p94), BRAF1, BRAF1MGC126806, BRaf, EC 2.7.11.1, MGC138284, NS7, Proto-oncogene B-Raf, RAFB1, murine sarcoma viral (v-raf) oncogene homolog B1, p94, serine/threonine-protein kinase B-raf, v-raf murine sarcoma viral oncogene homolog B1FLJ95109

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Recombinant Human B-Raf kinase domain His-tag Protein, CF New

R&D Systems, part of Bio-Techne | Catalog # 11831-BR

Key Product Details

Product Specifications for Recombinant Human B-Raf kinase domain His-tag Protein, CF

Scientific Data Examples for Recombinant Human B-Raf kinase domain His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: B-Raf

Customer Reviews for Recombinant Human B-Raf kinase domain His-tag Protein, CF

Product Documents for Recombinant Human B-Raf kinase domain His-tag Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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