Product Specifications for Recombinant Human AMIGO Protein, CF
Mouse myeloma cell line, NS0-derived human AMIGO protein Gly28-His367, with a C-terminal 6-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Starts at Gly28
Predicted Molecular Mass
50-65 kDa, reducing conditions
Measured by its ability to enhance survival of E16-E18 rat embryonic cortical neurons.
The ED50 for this effect is 0.6-2.4 μg/mL.
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth is observed between 1.25-10 μg/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
AMIGO (amphoterin-induced gene and ORF) is an approximately 75 kDa transmembrane cell adhesion protein. It belongs to a family of leucine‑rich repeat (LRR) containing proteins that play various roles in nervous system development and function (1). Mature human AMIGO consists of a 345 amino acid (aa) extracellular domain (ECD) with six tandem LRRs flanked by LRR N- and C‑terminal domains, one immunoglobulin‑like domain, a 21 aa transmembrane segment, and a 100 aa cytoplasmic domain (2). Within the ECD, human AMIGO shares 86% aa sequence identity with mouse and rat AMIGO. AMIGO is an N‑glycosylated protein that forms homodimers by association between LRR regions (3, 4). It interacts homophilically as well as heterophilically with the related AMIGO2 and AMIGO3 proteins (2). AMIGO is expressed along developing and mature neuronal fiber tracts on neurons, astrocytes, and oligodendrocytes (2, 4). It promotes the extension and branching of hippocampal and cortical neurons (2, 4). It also supports neuronal survival following apoptotic stimulation (4). AMIGO directly associates with the potassium channel Kv2.1 and enhances ion conductance through the channel (5). Kv2.1 localization and activation is regulated by phosphorylation, but phosphorylation status does not alter its interaction with AMIGO (5, 6).
de Wit, J. et al. (2011) Annu. Rev. Cell Dev. Biol. 27:697.
Kuja-Panula, J. et al. (2003) J. Cell Biol. 160:963.
Kajander, T. et al. (2011) J. Mol. Biol. 413:1001.
Chen, Y. et al. (2012) J. Cell. Physiol. 227:2217.
Peltola, M.A. et al. (2011) EMBO Rep. 12:1293.
Park, K.-S. et al. (2006) Science 313:976.
Amphoterin-induced Gene and ORF
Entrez Gene IDs
57463 (Human); 229715 (Mouse)
AMIGO, AMIGO-1, AMIGO1, AMIGOamphoterin-induced protein 1, Alivin-2, KIAA1163, adhesion molecule with Ig-like domain 1, ali2, amphoterin-induced gene and ORF
Citations for Recombinant Human AMIGO Protein, CF
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Product Documents for Recombinant Human AMIGO Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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