Recombinant Human AMIGO Protein, CF

Catalog # 2565-AM | R&D Systems, Inc. a Bio-Techne Brand
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2565-AM-050
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Key Product Details

Accession #

Q86WK6

Source

NS0

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human AMIGO protein
Gly28-His367, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Starts at Gly28

Predicted Molecular Mass

39.5 kDa

SDS-PAGE

50-65 kDa, reducing conditions

Activity

Measured by its ability to enhance survival of E16-E18 rat embryonic cortical neurons.

The ED50 for this effect is 0.6-2.4 μg/mL.



Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Optimal neurite outgrowth is observed between 1.25-10 μg/mL. 

Formulation, Preparation and Storage

2565-AM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AMIGO

AMIGO (amphoterin-induced gene and ORF) is an approximately 75 kDa transmembrane cell adhesion protein. It belongs to a family of leucine‑rich repeat (LRR) containing proteins that play various roles in nervous system development and function (1). Mature human AMIGO consists of a 345 amino acid (aa) extracellular domain (ECD) with six tandem LRRs flanked by LRR N- and C‑terminal domains, one immunoglobulin‑like domain, a 21 aa transmembrane segment, and a 100 aa cytoplasmic domain (2). Within the ECD, human AMIGO shares 86% aa sequence identity with mouse and rat AMIGO. AMIGO is an N‑glycosylated protein that forms homodimers by association between LRR regions (3, 4). It interacts homophilically as well as heterophilically with the related AMIGO2 and AMIGO3 proteins (2). AMIGO is expressed along developing and mature neuronal fiber tracts on neurons, astrocytes, and oligodendrocytes (2, 4). It promotes the extension and branching of hippocampal and cortical neurons (2, 4). It also supports neuronal survival following apoptotic stimulation (4). AMIGO directly associates with the potassium channel Kv2.1 and enhances ion conductance through the channel (5). Kv2.1 localization and activation is regulated by phosphorylation, but phosphorylation status does not alter its interaction with AMIGO (5, 6).

References

  1. de Wit, J. et al. (2011) Annu. Rev. Cell Dev. Biol. 27:697.
  2. Kuja-Panula, J. et al. (2003) J. Cell Biol. 160:963.
  3. Kajander, T. et al. (2011) J. Mol. Biol. 413:1001.
  4. Chen, Y. et al. (2012) J. Cell. Physiol. 227:2217.
  5. Peltola, M.A. et al. (2011) EMBO Rep. 12:1293.
  6. Park, K.-S. et al. (2006) Science 313:976.

Long Name

Amphoterin-induced Gene and ORF

Alternate Names

ali2, Alivin-2, AMIGO1

Entrez Gene IDs

57463 (Human); 229715 (Mouse)

Gene Symbol

AMIGO1

UniProt

Q86WK6

Product Documents for Recombinant Human AMIGO Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human AMIGO Protein, CF

For research use only

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