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Recombinant Human ALDH1A2 His-tag, CF

Bio-Techne includes R&D Systems | Catalog # 10132-DH

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10132-DH-020

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

E. coli-derived human ALDH1A2 protein
Thr2-Ser518
with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1

Predicted Molecular Mass

58 kDa

SDS-PAGE

58 kDa, under reducing conditions

Activity

Measured by the ability to catalyze the oxidation of 4-nitrobenzaldehyde.
The specific activity is >150 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human ALDH1A2 His-tag, CF

Recombinant Human ALDH1A2 His-tag SDS-PAGE

Recombinant Human ALDH1A2 His-tag SDS-PAGE

1 μg/lane of Recombinant Human ALDH1A2 His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing a band at 58 kDa under reducing conditions.

Formulation, Preparation and Storage

10132-DH
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: ALDH1A2

Aldehyde dehydrogenases (ALDHs) are NAD(P)+-dependent enzymes that detoxify aldehydes by oxidizing them to carboxylic acids. Nineteen ALDHs are present in humans, expressed in a variety of organelles and having different substrate preferences (1). Human ALDH1A2 is a NAD-dependent, cytosolic member of the ALDH1A subfamily class 1 that forms active tetramers that synthesize retinoic acid from retinal.  Each monomer has three functional regions: a catalytic domain containing the active site cysteine, an NAD-binding domain, and the oligomerization domain (2, 3). ALDH1A2 exhibits the highest substrate specificity and catalytic efficiency for retinal oxidation to retinoic acid (RA) (4) and thus plays an important role in regulation of RA production and signaling. ALDH1A2 null mice are embryonic lethal (5) and mutations in ALDH1A2 are associated with pathological conditions such as osteoarthris (6-8). Additionally, ALDH1A2 is suggested to play a role in several cancers. ALDH1A2 was a suggested tumor marker (9, 10) and predictor of prostate cancer relapse (11). Abnormally low levels of ALDH1A2 has been observed in several cancers including breast (12), squamous cell carcinoma of the head and neck (13), and specifically in high grade ovarian cancer, suggesting high expression may be associated with favorable prognosis (14).  Overexpression of ALDH1A2 in cancer lines resulted in decreased proliferation and migration (14) supporting that ALDH1A2 plays a tumor suppressor role. In contrast, ALDH1A2 is reported to exhibit high expression correlated with worse overall survival in non-small-cell lung cancer (15) and chemoresistant cancer stem cells in neuroblastoma (16, 17). Pharmacologically specific inhibitors and activators of ALDH1A2 are of interest due to its implied role in a variety of diseases and proliferation and drug resistance (1, 3, 15, 18).

References

  1. Koppaka, V. et al. (2012) Pharmacol. Rev. 64:520.
  2. Perez-Miller, S. J. and T. D. Hurley (2003) Biochemistry. 42:7100.
  3. Chen, Y. et al. (2018) ACS Chem. Biol. 13:582.
  4. Duester, G. (2001) Chem. Biol. Interact. 130:469.
  5. Niedereither, K. et al. (1999) Nat. Genet. 21:444.
  6. Pavan, M. et al. (2009) B.M.C. Med. Genet. 10:113.
  7. Styrkarsdottir, U. et al. (2014) Nat. Genet. 46:498.
  8. Shepherd, C. et. al. (2018) Arthritis Rheumatol. 70:1577.
  9. Kim, H. et al. (2005) Cancer Res. 65:8118.
  10. Touma, S. E. et al. (2009) Biochem. Pharmacol. 78:1127.
  11. Nim, H. T. et al. (2017) Front. Oncol. 7:30.
  12. Mira, Y. L. R. et al. (2000) J. Cell Physiol. 185:302.
  13. Seidensaal, K. et al. (2015) Mol. Cancer 14:204.
  14. Wang, Y. et al. (2018) Onco. Targets Ther. 31:599.
  15. You, Q. et al. (2015) Drug Des. Devel. Ther. 9:5087.
  16. Hartomo, T. B. et al. (2015) Int. J. Oncol. 46:1089.
  17. Singh, S. et al. (2015) Adv. Exp. Med. Biol. 815:281.
  18. Moreb, J. S. et al. (2012) Chem. Biol. Interact. 195:52.

Long Name

Aldehyde Dehydrogenase 1 Family, Member A2

Alternate Names

ALDH 1A2, RALDH, RALDH2

Entrez Gene IDs

8854 (Human); 19378 (Mouse)

Gene Symbol

ALDH1A2

UniProt

Additional ALDH1A2 Products

Product Documents for Recombinant Human ALDH1A2 His-tag, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human ALDH1A2 His-tag, CF

For research use only

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