Key Product Details
Structure / Form
Ile24-Ser247, with a C-terminal 10-His tag
N-terminal Sequence Analysis
Predicted Molecular Mass
The specific activity is >4,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
|Formulation||Supplied as a 0.2 μm filtered solution in HCl, NaCl, CaCl2 and Brij-35.|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Trypsin 3/PRSS3
Human Trypsin 3, encoded by the PRSS3 gene, is also known as mesotrypsin (1). Constituting less than 10% of the total trypsin content in normal pancreatic juice, it is one of the three trypsin isoforms produced by the pancreas (2). Compared to trypsin 1 and 2, one intriguing feature of Trypsin 3 is its resistance to polypeptide trypsin inhibitors, such as the Kunitz-type soybean trypsin inhibitor or the Kazal-type pancreatic secretory trypsin inhibitor. As revealed by the crystal structure, this resistance is likely due to the presence of an arginine residue in place of the highly conserved Gly198 (3). Trypsin 3 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. One proposed physiological function of Trypsin 3 is degradation of trypsin inhibitors, which facilitates the digestion of those foods rich in these proteins (4).
- Nyaruhucha, C. N. M. et al. (1997) J. Biol. Chem. 272:10573.
- Rinderknecht, H. et al. (1984) Gastroenterology 86:681.
- Katona, G. et al. (2002) J. Mol. Biol. 315:1209.
- Szmola, R. et al. (2003) J. Biol. Chem. 278:48580.
Product Specific Notices for Recombinant Human Active Trypsin 3/PRSS3 Protein, CF
For research use only