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Recombinant Human Active Trypsin 3/PRSS3 Protein, CF Best Seller
Catalog # 3714-SE | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Accession #
Source
NS0
Structure / Form
Active form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Trypsin 3/PRSS3 protein
Ile24-Ser247, with a C-terminal 10-His tag
Ile24-Ser247, with a C-terminal 10-His tag
Purity
>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ile24
Predicted Molecular Mass
26 kDa
SDS-PAGE
20-24 kDa, non-reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >4,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >4,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
3714-SE
| Formulation | Supplied as a 0.2 μm filtered solution in HCl, NaCl, CaCl2 and Brij-35. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Trypsin 3/PRSS3
Human Trypsin 3, encoded by the PRSS3 gene, is also known as mesotrypsin (1). Constituting less than 10% of the total trypsin content in normal pancreatic juice, it is one of the three trypsin isoforms produced by the pancreas (2). Compared to trypsin 1 and 2, one intriguing feature of Trypsin 3 is its resistance to polypeptide trypsin inhibitors, such as the Kunitz-type soybean trypsin inhibitor or the Kazal-type pancreatic secretory trypsin inhibitor. As revealed by the crystal structure, this resistance is likely due to the presence of an arginine residue in place of the highly conserved Gly198 (3). Trypsin 3 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. One proposed physiological function of Trypsin 3 is degradation of trypsin inhibitors, which facilitates the digestion of those foods rich in these proteins (4).
References
- Nyaruhucha, C. N. M. et al. (1997) J. Biol. Chem. 272:10573.
- Rinderknecht, H. et al. (1984) Gastroenterology 86:681.
- Katona, G. et al. (2002) J. Mol. Biol. 315:1209.
- Szmola, R. et al. (2003) J. Biol. Chem. 278:48580.
Alternate Names
Mesotrypsin, MTG, PRSS3, PRSS4, Trypsin 4, Trypsinogen 15
Gene Symbol
PRSS3
UniProt
Product Documents for Recombinant Human Active Trypsin 3/PRSS3 Protein, CF
Product Specific Notices for Recombinant Human Active Trypsin 3/PRSS3 Protein, CF
For research use only
Citations for Recombinant Human Active Trypsin 3/PRSS3 Protein, CF (2)
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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Active Trypsin 3/PRSS3 Protein, CF include:
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Species: Odorrana livida
Sample Types: Peptide
Applications: BioassayJ Yang et al. (2022-04-12), Engineering and Structural Insights of a Novel BBI-like Protease Inhibitor Livisin from the Frog Skin Secretion Toxins, 2022-014(4).
PMID: 35448882 -
Sample Types: Recombinant Protein
Applications: Enzyme AssayBirukawa N et al. (2014-05-27), Activated hepatic stellate cells are dependent on self-collagen, cleaved by membrane type 1 matrix metalloproteinase for their growth. J Biol Chem, 2014-0289(29):20209-21.
PMID: 24867951
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