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Recombinant Human Active Trypsin 3/PRSS3 Protein, CF Best Seller

Catalog # 3714-SE | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
Size / Price

Key Product Details

Accession #



Structure / Form

Active form




Enzyme Activity

Product Specifications


Mouse myeloma cell line, NS0-derived human Trypsin 3/PRSS3 protein
Ile24-Ser247, with a C-terminal 10-His tag


>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis


Predicted Molecular Mass

26 kDa


20-24 kDa, non-reducing conditions


Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >4,000 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

Formulation Supplied as a 0.2 μm filtered solution in HCl, NaCl, CaCl2 and Brij-35.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Trypsin 3/PRSS3

Human Trypsin 3, encoded by the PRSS3 gene, is also known as mesotrypsin (1). Constituting less than 10% of the total trypsin content in normal pancreatic juice, it is one of the three trypsin isoforms produced by the pancreas (2). Compared to trypsin 1 and 2, one intriguing feature of Trypsin 3 is its resistance to polypeptide trypsin inhibitors, such as the Kunitz-type soybean trypsin inhibitor or the Kazal-type pancreatic secretory trypsin inhibitor. As revealed by the crystal structure, this resistance is likely due to the presence of an arginine residue in place of the highly conserved Gly198 (3). Trypsin 3 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. One proposed physiological function of Trypsin 3 is degradation of trypsin inhibitors, which facilitates the digestion of those foods rich in these proteins (4).


  1. Nyaruhucha, C. N. M. et al. (1997) J. Biol. Chem. 272:10573.
  2. Rinderknecht, H. et al. (1984) Gastroenterology 86:681.
  3. Katona, G. et al. (2002) J. Mol. Biol. 315:1209.
  4. Szmola, R. et al. (2003) J. Biol. Chem. 278:48580.

Alternate Names

Mesotrypsin, MTG, PRSS3, PRSS4, Trypsin 4, Trypsinogen 15

Entrez Gene IDs

5646 (Human); 22073 (Mouse); 362347 (Rat)

Gene Symbol



Product Documents for Recombinant Human Active Trypsin 3/PRSS3 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Active Trypsin 3/PRSS3 Protein, CF

For research use only

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