E. coli-derived guinea pig Asparaginase protein Ala2-Ile565 with an N-terminal Met and 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
57 kDa, under reducing conditions
Measured by its ability to convert asparagine to aspartic acid. The specific activity is >2700 pmol/min/μg, as measured under the described conditions.
Recombinant Guinea Pig Asparaginase His-tag Protein, CF Scientific Data Examples
Recombinant Guinea Pig Asparaginase His-tag Protein Binding Activity
Recombinant Guinea Pig ASPG His-tag (Catalog # 10238-AS) is measured by its ability to convert asparagine to aspartic acid.
Recombinant Guinea Pig Asparaginase His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Guinea Pig Asparaginase His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band under reducing conditions at 57 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in Tris, NaCl and TCEP.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after opening.
L-Asparaginases catalyze the conversion of L-asparagine (Asn) to L-aspartic acid (Asp) and play important roles in amino acid metabolism of significance in medical, antimicrobial, and food industry applications (1). Guinea pig ASPG (gpASPG), also known as ASNase1, is a tetramer composed of a dimer of dimers. Each protomer contains a N-terminal domain with the active site and a C-terminal domain that can act as a lid when substrate is bound (2). gpASPG was identified as an L-asparaginase capable of inhibiting tumor growth (3, 4). It was observed that unlike most normal cells, in acute lymphatic leukemia (ALL) there is little or no detectable asparagine synthetase making cancer cells specifically sensitive to extracellular asparagine depletion with asparaginase treatment (5-7). Although bacterial asparaginases are currently available as therapeutics in ALL (8), treatment using bacterial origin asparaginase leads to immunogenic intolerance and clearance issues from the blood (9). Additionally, the bacterial asparaginases also show an unnecessary side L-glutaminase activity (10). For these reasons, a human-like enzyme would be preferred for therapeutic use (9, 11) but must have efficient hydrolysis of extracellular Asn with a Km value in the low micromolar range (2). Human ASPG is unable to match the required efficiency for therapeutic use (2, 12, 13), but gpASPG is homologous and has similar kinetic efficiency to the bacterial enzymes currently being used as therapeutics (2, 14). gpASPG may consequently make an appropriate mammalian-source therapeutic for replacement. There is significant focus on asparaginases given the broad beneficial applications for these enzymes.
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Entrez Gene IDs
374569 (Human); 100723883 (Guinea Pig)
ASPG, AnsA, AnsB, Asparaginase, C14orf76, Colaspase, Cytoplasmic asparaginase I, L ASNase I, L ASNase II, L asparaginase 1, L asparaginase 2, L asparaginase I, L asparaginase II, L asparaginase II precursor, L asparagine amidohydrolase I, L asparagine amidohydrolase II
Citations for Recombinant Guinea Pig Asparaginase His-tag Protein, CF
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