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Recombinant F. meningosepticum Endo F3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5548-GH

R&D Systems, part of Bio-Techne
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5548-GH-010

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

E. coli-derived f. meningosepticum Endo-beta-N-acetylglucosaminidase F3/Endo F3 protein
Ala44-Asn329, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

32 kDa

SDS-PAGE

30 kDa, reducing conditions

Activity

Measured by its ability to deglycosylate TSLP under native conditions.
The DC50 is <12 ng. The DC50 is defined as the amount of enzyme required to remove 50% of glycan on 1 μg of recombinant mouse TSLP in 30 minutes at 37 °C. . Use of Recombinant F. meningosepticum Endo‑ beta‑N‑acetylglucosaminidase F3/Endo F3 in the delycoslyation of other substrates may require alternative conditions for optimal performance.

Formulation, Preparation and Storage

5548-GH
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and EDTA.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Endo-beta-N-acetylglucosaminidase F3/Endo F3

N-glycans are commonly found on various glycoproteins. While peptide N-glycosidase from Flavobacterium meningosepticum (PNGase F) is widely used to release virtually all types of N-glycans under denaturing conditions, endo-beta -N-acetylglucosaminidases from the same bacterial species can be used under native conditions to specifically release particular types of N-glycans (1, 2). Because these glycosidases hydrolyze the chitobiose core of N-glycans, the released glycan products will contain one GlcNAc residue at their reducing ends with the other GlcNAc residue remaining attached to the asparagine residue on the glycoproteins. Endo F3 hydrolyzes bi- and triantennary N-glycans (3). In general, this enzyme is more efficient on core fucosylated N-glycans increasing activity up to 400-fold with biantennary structures (4). It also cleaves fucosylated trimannosyl core structures.

References

  1. Maley, F. et al. (1989) Anal. Biochem. 180:195.
  2. Tarentino, A.L. et al. (1985) Biochemistry 24:4665.
  3. Tarentino, A.L. et al. (1993) J. Biol. Chem. 268: 9702.
  4. Tarentino, A.L. and Plummer, T.H. Jr. (1994) Glycobiology 4:771.

Alternate Names

Endo F3, EndobetaNacetylglucosaminidase F3

UniProt

Additional Endo-beta-N-acetylglucosaminidase F3/Endo F3 Products

Product Documents for Recombinant F. meningosepticum Endo F3 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant F. meningosepticum Endo F3 Protein, CF

For research use only

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