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Recombinant F. graminearum Galactose Oxidase His Protein, CF

Bio-Techne includes R&D Systems | Catalog # 11206-GX

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11206-GX-050

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

E. coli-derived f. graminearum Galactose Oxidase protein
Ala42-Gln680, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala42

Predicted Molecular Mass

70 kDa

SDS-PAGE

60-68 kDa, under reducing conditions.

Activity

Measured by its ability to oxidize galactose.
The specific activity is >75,000 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant F. graminearum Galactose Oxidase His Protein, CF

Enzyme Reaction Scheme

Recombinant F. graminearum Galactose Oxidase His-tag Protein (Catalog # 11206-GX) oxidizes free and terminal D-Galactose to 6-Aldehyde-D-Galactose with the concomitant production of H2O2 that allows convenient detection through horseradish peroxidase.

Recombinant F. graminearum Galactose Oxidase His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant F. graminearum Galactose Oxidase His-tag Protein (Catalog # 11206-GX) was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 65 kDa.

Formulation, Preparation and Storage

11206-GX
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Galactose Oxidase

Fusarium graminearum galactose oxidase (GalOx) is an extracellular copper-containing oxidoreductase. Its catalytic site contains a free radical ligand formed by covalently cross-linked cysteine and tyrosine side chains coordinating to a copper center (1, 2, 3). GalOx catalyzes the two-electron oxidation of the C6-hydroxyl group of D-galactose as well as a range of primary alcohols to the corresponding aldehydes with concomitant reduction of oxygen to hydrogen peroxide. GalOx is strictly regioselective on primary alcohols and displays remarkable stereospecificity in its reaction with sugars, showing activity only with galactose but not glucose. Because of this specificity, GalOx is used to determine lactose levels in biological fluids, human milk and dairy products and examine histochemistry of mucus-secreting cells (4, 5). Furthermore, GalOx has been used to modify cell surface carbohydrates for cell labeling and histochemical staining and induce interferon in human lymphocyte culture (2, 6). GalOx is viewed as a competitive and cost-effective catalyst compared to chemical conversion for manufacturing fine chemicals in pharmaceutical and food industry. For example, GalOx is used for conversion of D-galactose to food-grade cross-linking agents (7).

References

  1. Baron, AJ. et al. (1994) J. Biol. Chem. 269:25095. 
  2. Whittaker, J.W. (2002) Adv. Protein Chem. 60:1.  
  3. Parikka K, et al.  (2015) J. Mol. Catal., B Enzym. 120:47. 
  4. Roberts, G.P. and Gupta, S.K. (1965) Nature 207:425. 
  5. Kanyong, P. et al. (2013) Anal. Biochem. 435:114. 
  6. Dianzani, F. et al.  Infect. Immun. (1979) 26:879. 
  7. Schoevaart, R. and Kieboom, T.  (2001) Carbohydr. Res. 334:1.

Entrez Gene IDs

23557903 (F. graminearum)

Gene Symbol

FGSG_11032

UniProt

Additional Galactose Oxidase Products

Product Documents for Recombinant F. graminearum Galactose Oxidase His Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant F. graminearum Galactose Oxidase His Protein, CF

For research use only

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