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Recombinant Cynomolgus/Rhesus EDAR Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10968-ER

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10968-ER-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived EDAR protein
Cynomolgus Monkey/Rhesus Macaque EDAR
(Glu27-Ala187)
Accession # XP_005575284.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Protein identity is confirmed by mass spectrometry.

Predicted Molecular Mass

44 kDa

SDS-PAGE

53-65 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human EDA-A1/Ectodysplasin A1 Protein (Catalog # 3944-ED) is immobilized at 1.00 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey/Rhesus Macaque EDAR Fc Chimera (Catalog # 10968-ER) that produces 50% of the optimal binding response is 1.50-15.0 ng/mL.

Scientific Data Images for Recombinant Cynomolgus/Rhesus EDAR Fc Chimera Protein, CF

Recombinant Cynomolgus Monkey/Rhesus Macaque EDAR Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque EDAR Fc Chimera Protein (Catalog # 10968-ER) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 53-65 kDa and 110-130 kDa, respectively.

Formulation, Preparation and Storage

10968-ER
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EDAR

EDAR is a type I transmembrane protein which is a member of the TNF Receptor Superfamily (TNFRSF). The extracellular domain contains 14 cysteine residues, six of which approximate the TNFRSF cysteine-rich region; the cytoplasmic domain contains a region with homology to the death domains found in other TNFRSF members. Based on its high homology with human EDAR, cynomologus EDAR is predicted to be a 488 amino acid (aa) protein with a 26 aa signal, a 163 aa extracellular domain, a 22 aa transmembrane domain, and a 277 aa cytoplasmic domain. The cynomolgus and human EDAR homologs share 99% identity. Within the TNFRSF, EDAR shares the highest homologies with XEDAR and TNFRSF19/TROY. EDA-A1 is the EDAR ligand. EDA and EDAR have been associated with hypohidrotic ectodermal dysplasia (HED). HED is characterized by abnormalities in hair, teeth and eccrine sweat gland morphogenesis. HED was initially found to associate with two gene loci, tabby and downless. Tabby was later identified as the gene for EDA and downless as the autosomal EDAR gene. EDA has two splice variants, EDA-A1 and EDA-A2, which differ by only two amino acids. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds EDAR, whereas EDA-A2 binds to XEDAR, an X-linked TNFRSF member with high homology to EDAR. Mutations in EDA, EDAR and XEDAR have been associated with HED.

References

  1. Headon, D.J. and P.A. Overbeek (1999) Nat.Genet. 22:370.
  2. Kumar, A. et al. (2000) J. Biol. Chem. 276:2668.
  3. Monreal, A.W. et al. (1999) Nat. Genet. 22:366.
  4. Schneider, P. et al. (2001) J. Biol. Chem. 276:18819.
  5. Srivastava, A.K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:13069.
  6. Yan, M. et al. (2000) Science 290:523.

Long Name

Ectodysplasin Receptor

Alternate Names

Anhidrotic ectodysplasin receptor 1, DLED3, Ectodermal dysplasia receptor, ectodysplasin 1, anhidrotic receptor, ectodysplasin A receptor, Ectodysplasin-A receptor, ED1R, ED5, EDA1R, EDA3EDA-A1 receptor, EDA-A1R, Edar, FLJ94390, HRM1, mouse, homolog of, tumor necrosis factor receptor superfamily member EDAR

Entrez Gene IDs

10913 (Human); 13608 (Mouse); 102120238 (Cynomolgus Monkey)

Gene Symbol

EDAR

UniProt

Additional EDAR Products

Product Documents for Recombinant Cynomolgus/Rhesus EDAR Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus/Rhesus EDAR Fc Chimera Protein, CF

For research use only

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