Human embryonic kidney cell, HEK293-derived cynomolgus monkey LDLR protein Ala22-Gly788, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
125-140 kDa, under reducing conditions.
Measured by its binding ability in a functional ELISA. When Human LDL is immobilized at 4.00 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey LDLR His-tag (Catalog # 111151-LD) binds with an ED50 of 30.0-300 ng/mL.
Recombinant Cynomolgus Monkey LDLR His-tag Protein, CF Scientific Data Examples
Recombinant Cynomolgus Monkey LDLR His-tag Protein Binding Activity.
When Human LDL is immobilized at 4.00 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey LDLR His-tag Protein (Catalog # 11151-LD) binds with an ED50 of 30.0‑300 ng/mL.
Recombinant Cynomolgus Monkey LDLR His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Cynomolgus Monkey LDLR His-tag Protein (Catalog # 11151-LD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 125-140 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 200 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
The low density lipoprotein receptor (LDLR) is the founding member of the LDLR family, a group of widely expressed type I transmembrane glycoprotein cell surface scavenger receptors (1-5). Members of the family are endocytic receptors which bind and internalize extracellular ligands, including lipoproteins, exotoxins, and lipid‑carrier complexes but can also co-regulate adjacent cell-surface signaling molecules (3, 4). Mature LDLR consists of an extracellular domain (ECD) with 7 cysteine‑rich complement-like repeats (class A LDL domains), calcium-binding EGF repeats, and beta ‑propeller structures (class B LDL repeats), a transmembrane domain and a cytoplasmic domain with an NPxY-repeat motif. The ECD of cynomologus LDLR shares 94% amino acid sequence identity with human LDLR. LDLR is constitutively and widely expressed and plays a key role in regulating cholesterol homeostasis. The class A LDL domains near the N-terminus of LDLR bind apoB and apoE, the apolipoproteins of low- and very low-density lipoproteins (LDL and VLDL), respectively (1, 2, 4, 7). Hepatocyte LDLR is responsible for endocytosis and clearing of most plasma LDL cholesterol from circulation (2, 7). At the low pH of the endocytic vesicle, it dissociates, allowing degradation of LDL and recycling of LDLR to the cell surface (1, 4). Lack of LDLR expression or function causes familial hypercholesterolemia (FH) and leads to premature cardiovascular disease (4, 7, 8). The protease PCSK9 (proprotein convertase subtilisin/kexin type 9) can also cause increased plasma cholesterol by promoting LDLR degradation rather than recycling to the cell surface (8-10). Additionally, soluble forms of LDLR are reported to be released by phorbol esters or interferons and could serve as a disease marker (5, 11, 12). Several studies have demonstrated a role for LDLR in cancer progression, including liver cancer, leukemia, lung cancer, breast cancer, colorectal cancer, and prostate cancer (13).
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Gent, J. and I. Braakman (2004) Cell. Mol. Life Sci. 61:2461.
Begg, M.J. et al. (2004) Eur. J. Biochem. 271:524.
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Zhang, D.W. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:13045.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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