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Recombinant Cynomolgus Monkey Fas/TNFRSF6/CD95 Fc, CF

Catalog # 10755-FS | R&D Systems, Inc. a Bio-Techne Brand
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10755-FS-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Fas/TNFRSF6/CD95 protein
Cynomolgus Monkey Fas/TNFRSF6/CD95
(Gln26-Asp173)
Accession # Q9TSN4.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln26, inferred from deblocking revealing Val27

Predicted Molecular Mass

43 kDa

SDS-PAGE

55-70 kDa, under reducing conditions

Activity

Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. Cheng, J. et al. (1994) Science 263:1759.
The ED50 for this effect is 40.0-240 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey Fas/TNFRSF6/CD95 Fc, CF

Recombinant Cynomolgus Monkey Fas/TNFRSF6/CD95 Fc Chimera Bioactivity.

Recombinant Cynomolgus Monkey Fas/TNFRSF6/CD95 Fc Chimera (Catalog # 10755-FS), inhibits Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 40.0-240 ng/mL.

Recombinant Cynomolgus Monkey Fas/TNFRSF6/CD95 Fc Chimera SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey Fas/TNFRSF6/CD95 Fc Chimera (Catalog # 10755-FS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-70 kDa and 110-140 kDa, respectively.

Formulation, Preparation and Storage

10755-FS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fas/TNFRSF6/CD95

Fas (fibroblast associated; also known as APO-1 or CD95) is a member of the death receptor subfamily of the TNF receptor superfamily and is designated TNFRSF6 (1-3). The human Fas precursor is 335 amino acids (aa) in length, and contains a 25 aa signal peptide, a 148 aa extracellular domain (ECD), a 17 aa transmembrane sequence, and a 145 aa cytoplasmic region. The ECD possesses three cysteine-rich TNFR repeats, while the cytoplasmic region contains one death domain (DD) that is required for the transduction of apoptotic signals (4). Cynomolgus monkey Fas ECD shares 91% aa sequence identity with human Fas ECD. A human Fas isoform of 314 aa that lacks the transmembrane sequence is secreted by resting lymphocytes, while isoforms of 149, 132, 103 and 86 aa that also lack the DD and show substitutions for parts of the TNFR repeats are less prominently expressed (4-6). All five isoforms block the extrinsic apoptosis pathway induced by Fas ligand binding. Fas ligand (FasL; also TNFSF6) is a type II transmembrane protein that belongs to the TNF family and is expressed on activated T-cells, NK cells, and cells found in immune privileged sites. Alternatively, FasL is also shed as a soluble form (2, 6). Engagement of FasL induces oligomerization of preformed Fas trimers (1, 2). This activated receptor complex recruits the adaptor molecule FADD to form the Death-Inducing Signaling Complex (DISC). Upon activation, caspases in the DISC initiate the apoptotic signaling cascade (7). Fas is prominent in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes and tumor cells. It is an essential mediator in the activation-induced death of T lymphocytes that terminates the immune reaction (1, 2, 8). In immune-privileged tissues, infiltrating Fas-bearing lymphocytes and inflammatory cells are killed by FasL engagement (9). Both humans and mice with genetic defects in Fas accumulate abnormal lymphocytes and develop systemic autoimmunity (1-3). The Fas pathway also appears to intersect with the BIM (mitochondrial/intrinsic) apoptosis pathway (1).

References

  1. Bouillet, P. and L.A. O’Reilly (2009) Nat. Rev. Immunol. 9:514.
  2. Strasser, A. et al. (2009) Immunity 30:180.
  3. Ashkenazi, A. and V. Dixit (1999) Curr. Opin. Cell Biol. 11:255.
  4. SwissProt Accession # P25445.
  5. Liu, C. et al. (1995) Biochem. J. 310:957.
  6. Papoff, G. et al. (1996) J. Immunol. 156:4622.
  7. Thorburn, A. (2003) Cellular Signaling 16:139.
  8. Barreiro, R. et al. (2004) J. Immunol. 173:1519.
  9. Ferguson, T.A. and T.S Griffith (2006) Immunol. Rev. 213:228.

Long Name

Fibroblast-associated

Alternate Names

Apo-1, APT1, CD95, TNFRSF6

Entrez Gene IDs

355 (Human); 14102 (Mouse); 246097 (Rat); 102140989 (Cynomolgus Monkey); 493881 (Feline)

Gene Symbol

FAS

UniProt

Product Documents for Recombinant Cynomolgus Monkey Fas/TNFRSF6/CD95 Fc, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus Monkey Fas/TNFRSF6/CD95 Fc, CF

For research use only

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