Mouse myeloma cell line, NS0-derived cynomolgus monkey CD39/ENTPD1 protein Thr45-Val485, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
60-77 kDa, under reducing conditions
Measured by its ability to hydrolyze the 5’-phosphate groups from the substrate adenosine-5’-triphosphate (ATP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996). The specific activity is >15,000 pmol/min/μg, as measured under the described conditions.
Recombinant Cynomolgus Monkey CD39/ENTPD1 His Protein, CF Scientific Data Examples
Recombinant Cynomolgus Monkey CD39/ENTPD1 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Cynomolgus Monkey CD39/ENTPD1 His-tag Protein (Catalog # 11092-EN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-77 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after opening.
CD39, also known as ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1), is the prototypical member of the NTPDase family. CD39 is a metal-dependent, homodimeric enzyme bound to the cell membrane through two transmembrane domains present at each termini (1-3). The extracellular loop contains two major domains with an active site and apyrase conserved regions (ACRs) and changes conformation dynamically during catalysis (1-4). Although first described as a B lymphocyte cell surface marker (5), CD39 is also present on the surface of natural killer cells, T cells, and some endothelial cells (6). CD39 hydrolyzes the beta - and gamma phosphate residues of nucleotides to terminate purinergic signaling and is uniquely capable of processive hydrolysis of extracellular ATP to AMP without release of the ADP intermediate (3, 7, 8) as the initial rate-limiting steps in the production of extracellular adenosine (9). CD39 functional activity leads to interest in CD39 as a pharmaceutical therapeutic target for several disease models including inflammation, cancer, and immunosuppression (3,4,9,10). Recombinant cynomolgus NTPDase-1 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble recombinant NTPDase-1 ectodomain.
Grinthal, A. and G. Guidotti. (2004) Biochemistry 43:13849.
Robson, S.C. et al. (2006) Purinergic Signal. 2:409.
Zebisch, M. et al. (2012) J. Mol. Biol. 415:288.
Zimmermann, H. (2021) Purinergic Signal. 17:117.
Rowe, M. et al. (1982) Int. J. Cancer 29:373.
Kansas, G.S. et al. (1991) J. Immunol. 146:2235.
Kishore, B.K. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F1032.
Laliberte, J. F. and A.R Beaudoin (1983) Biochim. Biophys. Acta 742:9.
Moesta, A.K. et al. (2020) Nat. Rev. Immunol. 20:739.
Augustin, R.C. et al. (2022) J. Immunother. Cancer. 10:e004089.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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