Source	Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey CD200 protein Gln56-Gly257, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Gln56
Predicted Molecular Mass	23 kDa
SDS-PAGE	38-46 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human CD200 R1 Fc Chimera (Catalog # 3414-CD) is immobilized at 2 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey CD200 binds with an ED₅₀ of 0.04-0.24 ng/mL.

Recombinant Cynomolgus Monkey CD200 His-tag Protein, CF Scientific Data Examples

	Recombinant Cynomolgus Monkey CD200 His-tag Protein Binding Activity When Recombinant Human CD200 R1 Fc Chimera (Catalog # 3414-CD) is coated at 2 µg/mL, 100 µL/well, Recombinant Cynomolgus Monkey CD200 (Catalog # 10086-CD) binds with an ED₅₀ of 0.04-0.24 µg/mL.
	Recombinant Cynomolgus Monkey CD200 His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Cynomolgus Monkey CD200 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 38-46 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10086-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD200

CD200, also known as OX-2, is a 45 kDa transmembrane immunoregulatory protein that belongs to the immunoglobulin superfamily (1). Mature cynomolgus CD200 consists of a 202 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain and one Ig-like C2-type domain, a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. Within the ECD, cynomolgus CD200 shares 95%, 78%, and 78% aa sequence identity with human, mouse, and rat CD200, respectively. CD200 is widely but not ubiquitously expressed (2). Its receptor (CD200 R1) is restricted primarily to mast cells, basophils, macrophages, and dendritic cells, which suggests myeloid cell regulation as the major function of CD200 (3-5). CD200 knockout mice are characterized by increased macrophage number and activation and are predisposed to autoimmune disorders (6). CD200 and CD200 R associate via their respective N-terminal Ig-like domains (7). In myeloid cells, CD200 R initiates inhibitory signals following receptor-ligand contact (4, 5, 8). In T cells, however, CD200 functions as a co-stimulatory molecule independent of the CD28 pathway (9). Several additional CD200 R-like molecules have been identified in human and mouse, but their capacity to interact with CD200 is controversial (10, 11). Several viruses encode CD200 homologs which are expressed on infected cells during the lytic phase (12, 13). Like CD200 itself, viral CD200 homologs also suppress myeloid cell activity, enabling increased viral propagation (3, 12-14).

Item	Value
References	Vaine, C.A. and R.J. Soberman (2014) Adv. Immunol. 121:191. Wright, G.J. et al. (2001) Immunology 102:173. Shiratori, I. et al. (2005) J. Immunol. 175:4441. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348. Fallarino, F. et al. (2004) J. Immunol. 173:3748. Hoek, R.M. et al. (2000) Science 290:1768. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191. Borriello, F. et al. (1997) J. Immunol. 158:4548. Gorczynski, R. et al. (2004) J. Immunol. 172:7744. Hatherley, D. et al. (2005) J. Immunol. 175:2469. Foster-Cuevas, M. et al. (2004) J. Virol. 78:7667. Cameron, C.M. et al. (2005) J. Virol. 79:6052. Langlais, C.L. et al. (2006) J. Virol. 80:3098.
Entrez Gene IDs	4345 (Human); 17470 (Mouse); 102146004 (Cynomolgus Monkey)
Alternate Names	CD200, CD200 antigenMOX1, CD200 molecule, MOX1, MOX2, MOX2MRC, MRC, MRC OX-2 antigen, OX-2, OX-2 membrane glycoprotein, antigen identified by monoclonal MRC OX-2

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Recombinant Cynomolgus Monkey CD200 His-tag Protein, CF

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