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Recombinant Cynomolgus Monkey CD200 His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10086-CD

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10086-CD-100

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey CD200 protein
Gln56-Gly257, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln56

Predicted Molecular Mass

23 kDa

SDS-PAGE

38-46 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD200 R1 Fc Chimera (Catalog # 3414-CD) is immobilized at 2 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey CD200 binds with an ED50 of 0.04-0.24 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey CD200 His-tag Protein, CF

Recombinant Cynomolgus Monkey CD200 His-tag Protein Binding Activity

Recombinant Cynomolgus Monkey CD200 His-tag Protein Binding Activity

When Recombinant Human CD200 R1 Fc Chimera (Catalog # 3414-CD) is coated at 2 µg/mL, 100 µL/well, Recombinant Cynomolgus Monkey CD200 (Catalog # 10086-CD) binds with an ED50 of 0.04-0.24 µg/mL.
Recombinant Cynomolgus Monkey CD200 His-tag Protein SDS-PAGE

Recombinant Cynomolgus Monkey CD200 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey CD200 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 38-46 kDa.

Formulation, Preparation and Storage

10086-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: CD200

CD200, also known as OX-2, is a 45 kDa transmembrane immunoregulatory protein that belongs to the immunoglobulin superfamily (1). Mature cynomolgus CD200 consists of a 202 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain and one Ig-like C2-type domain, a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. Within the ECD, cynomolgus CD200 shares 95%, 78%, and 78% aa sequence identity with human, mouse, and rat CD200, respectively. CD200 is widely but not ubiquitously expressed (2). Its receptor (CD200 R1) is restricted primarily to mast cells, basophils, macrophages, and dendritic cells, which suggests myeloid cell regulation as the major function of CD200 (3-5). CD200 knockout mice are characterized by increased macrophage number and activation and are predisposed to autoimmune disorders (6). CD200 and CD200 R associate via their respective N-terminal Ig-like domains (7). In myeloid cells, CD200 R initiates inhibitory signals following receptor-ligand contact (4, 5, 8). In T cells, however, CD200 functions as a co-stimulatory molecule independent of the CD28 pathway (9). Several additional CD200 R-like molecules have been identified in human and mouse, but their capacity to interact with CD200 is controversial (10, 11). Several viruses encode CD200 homologs which are expressed on infected cells during the lytic phase (12, 13). Like CD200 itself, viral CD200 homologs also suppress myeloid cell activity, enabling increased viral propagation (3, 12-14).

References

  1. Vaine, C.A. and R.J. Soberman (2014) Adv. Immunol. 121:191.
  2. Wright, G.J. et al. (2001) Immunology 102:173.
  3. Shiratori, I. et al. (2005) J. Immunol. 175:4441.
  4. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
  5. Fallarino, F. et al. (2004) J. Immunol. 173:3748.
  6. Hoek, R.M. et al. (2000) Science 290:1768.
  7. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
  8. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
  9. Borriello, F. et al. (1997) J. Immunol. 158:4548.
  10. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
  11. Hatherley, D. et al. (2005) J. Immunol. 175:2469.
  12. Foster-Cuevas, M. et al. (2004) J. Virol. 78:7667.
  13. Cameron, C.M. et al. (2005) J. Virol. 79:6052.
  14. Langlais, C.L. et al. (2006) J. Virol. 80:3098.

Alternate Names

CD200, MOX1, MOX2, MRC, OX-2

Entrez Gene IDs

4345 (Human); 17470 (Mouse); 102146004 (Cynomolgus Monkey)

Gene Symbol

CD200

UniProt

Additional CD200 Products

Product Documents for Recombinant Cynomolgus Monkey CD200 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus Monkey CD200 His-tag Protein, CF

For research use only

Reconstitution Calculator

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